The application of biological agents can be done singly or in combination to increase its effectiveness. Trichoderma sp. fungi, Bacillus sp. and fluorescent psudomonads bacteria has been widely used as a biological control agent for plant pathogens. The purpose of this study was to determine the growth and reproduction of Trichoderma sp. in the presence of Bacillus sp., or fluorescent psudomonads. The research was carried out in vitro by dual and double layer culture method. The variables observed were the inhibition zone, the total and, viability of conidia of Trichoderma sp. The data obtained were compared using the two free sample T test and analysis of variance was continued with Duncan’s multiple range test. Based on the results of this study concluded that the growth and reproduction of Trichoderma sp inhibited by the presence of Bacillus sp. or fluorescent psudomonads, but does not reduce the viability of conidia. This shows that in Trichoderma sp. with Bacillus sp. or fluorescent psudomonads can be applied simultaneously.