Green Betel (Piper betle L.) leaves are often used as medicine by local people because they contain antibacterial and antifungal substances. These substances can be produced by plant metabolites and are also the results of metabolites produced by endophytic fungi. Endophytic fungus is an organism that is associated with healthy host tissues without causing disease symptoms. Endophytic fungi from betel leaf are used as biocontrol agents against Fusarium oxysporum causes wilt disease that attacks Chili (Capsicum annuum L.), which is one of the main agricultural commodities in Indonesia. Fusarium wilt disease is a disease that significantly reduces crop yields based on crop yields. This research was conducted in a chili farm in Cangkringan, Sleman, Yogyakarta. This study aimed to test the antagonism of endophytic fungi with the dual culture technique. Three types of green betel leaf endophytic fungi were used for dual culture test against Fusarium oxysporum. The observation result showed the highest inhibition ability of 73.37% dual culture method. The inhibitory mechanisms of green betel leaf endophytic fungal isolates include parasitism, antibiosis, and competition. Endophytic fungi of green betel leaf are effective as biocontrol agents of wilt Fusarium chili disease. ©2022 JNSMR UIN Walisongo. All rights reserved.
In the processing of chips using cassava (Manihot utilissima), it will produce waste in the form of solid and liquid waste. The liquid waste of processing cassava chips contains organic compounds that are easily decomposed and cause unpleasant odors. If the liquid waste is discharged directly into the environment, it will cause pollution and damage the environment. One of the steps that can be used to overcome this problem is phytoremediation. The purpose of this study was to determine the best treatment in the processing of cassava chips industrial wastewater so that the liquid waste is safe to dispose of into the environment. In this study, using two types of aquatic plants, namely water hyacinth (Eichhornia crassipes) and kiambang (Pistia stratiotes) and using alum to remediate liquid waste. This research was conducted experimentally using a jar with a diameter of 15 cm and a height of 25 cm with 3 repetitions of each treatment. The data obtained were analyzed descriptively to determine the best treatment that can be used for remediation of cassava chips industry wastewater. The results showed that the use of water hyacinth plants (Eichhornia crassipes) could improve the quality of wastewater so that it is safe to dispose of into the environment.
This research aims to determine the potential of bacterial isolates from Marina beach seawater to be used as natural textile dyes and to test their antibacterial ability. The media used for culturing bacteria is a zobel synthesis medium with a mixture of seawater. The results of the isolation and extraction of color pigments obtained a red color with a characteristic wavelength of 535 nm and has a variety of chemical content results. Pigment immersion trials using 3 types of fabric, namely cotton, primisima and dobby fabric. The results of immersion with ethanolic solvent obtained optimum results for 12 hours and with the addition of mordant (Fe.H2O4S.7H2O), the color of the fabric is strong enough to withstand washing with detergent and sunlight. From the results of the study, data showed that the dyed primisima fabric gave a reduction or inhibition of the growth of E. coli bacteria by 9% while the inhibition of S. aureus bacteria was 116%. The pigment has the potential to be used as a dye for batik cloth with the provisions of optimizing to find the right reinforcement or mordant. This study has implications for the discovery of red pigment-producing bacteria from the isolation of seawater at Marina Semarang beach.
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