Mimusops elengi, commonly called Bakul, is a medicinal plant belonging to the family Sapotaceae. It is a small to large evergreen tree up to 15 m in height. All parts of the tree have medicinal properties. The bark, flowers, and fruits are acrid, astringent, cooling, and anthelmintic [1]. The bark is used as a tonic [1][2][3][4], febrifuge, and as a gargle for odontopathy, inflammation, and bleeding of gums [1]. The powder of dried flower is a brain tonic and is useful as snuff to relieve cephalalgia. Young twigs are used for cleaning teeth [2]. It is antipyretic and increases fertility in women [1,3]. It is also useful in urethrorrhea, cystorrhea, diarrhea, and dysentery. Flowers are used for preparing lotion for wounds and ulcers [3]. The unripe fruit is used as a masticatory and helps to fix loose teeth. Seeds are used for preparing suppositories to treat constipation, especially in children [2][3][4]. Ripe fruit pulp is useful in chronic dysentery [3,4]. Leaves are used in snake bite [3,4].In our continuing research on bioactive metabolites of plant origin, we have isolated dibutyl phthalate (1) for the first time from the bark of Mimusops elengi. It has been also isolated from other plants, marine algae, bacteria, and fungi [5][6][7][8]. The cambridge Crystallographic Database has been searched for single-crystal analysis. So far, the molecular structure for compound 1 has not been determined; however, the manganese complex of the same has been studied by D. Moon and M. SooLah [9]. General Comments. Instrument: HPLC (Shimadzu 2010, LC Solution); LC-MS (Shimadzu 2010, LC-MS). Solution. Instrumental conditions: HPLC column YMC ODC -A (5 cm u 4.5 cm u 5P), A = 0.1% TFA in H 2 O, B = 0.1% TFA in acetonitrile, gradient = 5% B to 90% B in 8 min hold for 2 min, injection volume 3 PL; column oven temperature 30qC; flow rate = 1.0 mL/min. LC-MS conditions: scan mode = positive; CDC temperature 250qC; heater block 200qC, detector voltage 1.5 kV; scan speed 1000 amu/sec; nebulizing gas flow 1.5 L/min; ionization mode ESI (atmospheric pressure ionization).Extraction and Isolation. Plant material used in this study was purchased from the market. It was authenticated at the Agharkar Research Institute, Pune, India. Its authentication number is AHMA S/B-065. Air shade dried powdered bark material (300 g) was extracted with a Soxhlet extractor using different solvents such as hexane, chloroform, ethanol, and methanol for 18 hours. The solvents were removed under reduced pressure to get the respective extracts. The chloroform extract (1.33%, 4.018 g) was further purified. Broad fractionation of the crude chloroform extract (4.0 g) was carried out using gradient polarity solvents on silica gel (60-120 mesh, 160 g) to get ten fractions. Fractions were monitored by thin layer chromatography. Fractions 3 and 4 (toluene, 100%, toluene-ethyl acetate, 75:25, 1, 2 g) were mixed together for repeated column chromatography using gradient polarity solvents on silica gel (60-120 mesh, 60 g), and a total of nine fractions was collected. ...

Herbs have been used for medicinal purposes for centuries. According to recent investigations, they may help reduce the risk of chronic diseases, cardiovascular disease, and cancer due to antioxidant properties, which in turn can be attributed to the various phytoconstituents. With this intention, evaluation of antioxidant activity was performed. Methanol extract of aerial parts of Artemisia pallens Wall was screened for its antioxidant activity due to phenolic and flavonoid contents, by employing radical scavenging assays; 2,2 –diphenyl, 1-picryl hydrazyl (DPPH) and nitric oxide. Ascorbic acid was used as a standard. Quantitative determination of phenols and flavonoids were carried out using spectrophotometric method. Total flavonoid content was determined as quercetin equivalent and total phenolic content was determined as pyrocatechol equivalent using Folin-Ciocalteu reagent. Plant produces more phenolic compounds than flavonoids. IC50 value of methanol extract for DPPH free radical scavenging activity was found to be 292.7 μg, whereas for nitric oxide it was 204.61 μg. The result obtained in the present study indicates that the aerial parts of this plant are a rich source of natural antioxidants