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A transitory hyperglycemia was observed after production of bile pancreatitis in the dog. This phenomenon was characterized by an immediate increase in blood sugar after injection of bile, reaching a maximum at 10–30 minutes and returning to or near normal values within 45–90 minutes. Substances other than bile, particularly vegetable oils, also produced this reaction. It was not changed significantly in adrenalectomized animals or in those pretreated with dihydroergotamine, but in cobalt-treated dogs the response was almost completely prevented. Epinephrine from the adrenals appears to be ruled out as the major causative factor, whereas glucagon is implicated. Dihydroergotamine did not completely block hyperglycemia following epinephrine in the dog under the conditions of our experiment.

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We describe a sensitive, precise assay of serum or urine amylase activity, with use of a new substrate, Procion Brilliant Red M-2BS—Amylopectin. After 0.2 ml of serum or urine is incubated with substrate for 10 min at 37°C, ethylene glycol monomethyl ether is added to precipitate proteins and larger substrate particles. Clarity and chromogenicity of the final solution are not sensitive to small changes in temperature or concentrations of the various reagents. No interferences necessitating preparation of specimen blanks have been encountered. Human salivary amylase, assayed by a reference saccharogenic method, is used for calibration. When read against the resulting curve, normal sera and urines give activities comparable to those obtained with the reference method. Human pancreatic extract, sera and urines from pancreatitis patients, and macroamylasemia serum show higher activities by the proposed method.