A serum‐free medium has been devised which permits proliferation of the mouse primitive nervous cell line F7. When cholesterol, eye‐derived growth factor and brain extract are added in this medium for 48 h, 80‐90% of oligodendrocyte‐like cells are generated. These cells have diminished substrate adhesion. They acquire the capacity to synthesize carbonic anhydrase II and myelin basic protein, two specific proteins of oligodendrocytes. These observations suggest that F7 clonal cell line, which has been previously shown to be a neurophysin cell precursor, is also a precursor for oligodendrocytes, and represents a bipotent stem cell line for both neuronal and glial cell lineages.

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BACKGROUNDAcute Kidney Injury (AKI) in intensive care unit (ICU) is very common. This study looked at the clinical profile of patients admitted in ICU with AKI. MATERIALS AND METHODSProspective observational study was done in an ICU of a tertiary care centre in Mangalore. Patients admitted to the ICU who were 18 years of age and had diagnosis of AKI by KDIGO criteria were included in the study. Data was collected and analysed. RESULTSTotal number of patients included in the study was 100. Diabetes mellitus was the most common comorbidity seen in 38% cases followed by Hypertension in 27%. The duration of ICU stay ranged from 4 days to 12 days. Sepsis was the most common cause of AKI (51%). Forty-five percent of patients required haemodialysis. CONCLUSIONSepsis was the most common aetiological factor responsible for AKI. Diabetes mellitus was found to be the most common co-morbid illness associated with development of acute kidney injury. Respiratory tract infection was found to be the most common diagnosis at admission among those with AKI. Most AKI in ICU have oliguria. Acute tubular necrosis was found to be the most common pathophysiological process responsible for AKI. Forty-five percent of ICU-AKI patients require RRT. SOFA score of ≥ 9 and increasing severity of AKI were associated with increased mortality and also poor rate of recovery from acute kidney injury and increase d mortality.