Aim: The aim of present study was to investigate the nutritional composition, phytochemical constituents and in vitro antioxidant activities of a wild edible fruit in order to document data for further basic research needs or to widen its use among common people. Methods: The fresh fruits of Grewia sapida Roxb. ex DC. were collected from the Chirang district of Bodoland Territorial Area Districts of Assam, identified and freeze dried. Proximate composition, minerals content and phytochemical constituents were analyzed following standard and reported methods. The antioxidant activities of methanol extract were evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-Azinobis (3-ethylbenothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays. Results: The results obtained were reported and discussed. The phytochemical screening of different solvent extracts revealed that fruits contained a number of medicinally active secondary metabolites. The study showed antioxidant properties having IC 50 value of 257.666 ± 2.516 μg/mL (DPPH) and 134.33 ± 4.041 μg/mL (ABTS). The total phenolic and flavonoid contents in G. sapida fruit were found to be 294.353 ± 4.696 mg GAE/g dried extract and 116.95 ± 10.71 mg QE/g dried extract respectively. Conclusion: The study suggests that G. sapida fruit could be a good source of minerals and natural antioxidants, and studied further for its beneficial effect in human health.
The purpose of present study was to investigate the nutritional composition, phytochemical contents and antioxidant capacities of two wild edible fruits viz. Eugenia operculata Roxb. and Antidesma bunius L. from Assam of North-East India. The fruits showed variable amounts of proximate and mineral compositions which are reported herein and discussed. The phytochemical screening of different solvent extracts exhibited the presence of many phytochemicals which are biologically important. The antioxidant activities were examined using DPPH (1, 1-diphenyl-2-picrylhydrazyl), ABTS (2, 2 -Azinobis (3ethylbenothiazoline-6-sulfonic acid) diammonium salt), H 2 O 2 (Hydrogen peroxide) and FRAP (Ferric reducing antioxidant power) assays. The fruits showed antioxidant properties with DPPH IC 50 value of 92.330 ± 4.163 g/mL (E. operculata) and 395.002 ± 3.605 g/mL (A. bunius), ABTS IC 50 value of 52.660 ± 1.154 g/mL (E. operculata) and 105.331 ± 3.055 g/mL (A. bunius), H 2 O 2 IC 50 value of 20.566 ± 0.208 g/mL (E. operculata) and 24.366 ± 0.057 g/mL (A. bunius), and FRAP value of 281.583 ± 8.799 M TE/g DE (dry extract) in E. operculata and 61.583 ± 3.818 M TE/g DE in A. bunius. The total phenolic content (TPC) in E. operculata and A. bunius fruits were found to be 226.741 ± 2.099 mg GAE/g DE and 119.356 ± 1.395 mg GAE/g DE, respectively, while the total flavonoid content (TFC) were 108.761 ± 7.015 mg QE/g DE and 64.323 ± 8.828 mg QE/g DE, respectively. The fresh fruits of A. bunius and E. operculata were found to contain vitamin C of 7.30 ± 1.452 mg/100 g and 6.60 ± 1.123 mg/100 g, respectively. The studies revealed that E. operculata fruit had stronger antioxidant activity than A. bunius fruit showing better DPPH, ABTS and H 2 O 2 scavenging activities, and higher FRAP value, TPC and TFC. TPC and TFC showed a strong positive correlation with antioxidant activity assayed by FRAP. A strong positive correlation of antioxidant activity (DPPH, ABTS and H 2 O 2 ) was also found with vitamin C.
The aim of this study was to examine the phytochemicals and antioxidant properties of eleven wild edible plants from Assam of North-East India. The phytochemical study indicated the presence of several medicinally active compounds in the methanolic extracts of plants. Evaluation of antioxidant activities were done by DPPH, ABTS, H 2 O 2 and FRAP assays. The investigation revealed antioxidant activities with DPPH IC 50 value ranging from 135.0 ± 1.49 g/mL (L. javanica) to 516.34 ± 2.52 g/mL (B. lanceolaria), ABTS IC 50 value from 74.3 ± 0.29 g/mL (T. angustifolium) to 437.77 ± 3.93 g/mL (D. cordata), H 2 O 2 IC 50 value from 20.37 ± 0.01 g/mL (B. lanceolaria) to 376.75 ± 14.12 g/mL (P. perfoliatum), and the FRAP value from 64.76 ± 7.43 M TE/g (D. cordata) to 799.28 ± 7.14 M TE/g (L. javanica). The maximum total phenolic content (TPC) was obtained in the extract of E. fluctuans (269.49 ± 2.96 mg GAE/g dry extract) and the lowest being in C. sinensis (26.96 ± 9.81 mg GAE/g dry extract). S. media extract had the lowest (0.23 ± 0.10 mg QE/g dry extract) total flavonoid content (TFC) and the maximum being in P. perfoliatum (4.34 ± 1.03 mg QE/g dry extract). Pearson's correlation study of the plants indicated a strong positive correlation of DPPH assay with ABTS assay. A positive correlation of FRAP with TFC, H 2 O 2 with FRAP, TPC and TFC, and TPC with TFC were also seen in this study. These plants could be supportive in stopping or slowing the growth of oxidative stress related diseases.
The aim of present study was to ascertain the nutritional properties, phytochemical contents and in vitro antioxidant capacities of two wild edible fruits viz. Aporosa dioica (Roxb.) Muell.-Arg. and Ottelia alismoides (L.) Pers. found in Assam of North-East India. Nutritional properties, phytochemical screening, total phenolic content (TPC) and total flavonoid content (TFC) were investigated employing standard methods. Antioxidant properties were assessed following DPPH (1, 1-diphenyl-2-picrylhydrazyl), ABTS (2, 2´-Azinobis (3-ethylbenothiazoline-6-sulfonic acid) diammonium salt), H2O2 (Hydrogen peroxide) and FRAP (Ferric reducing antioxidant power) assays. The results obtained in this study were presented and discussed. A. dioica fruit extract exhibited lower IC50 (DPPH, ABTS and H2O2) values in contrast to O. alismoides fruit extract indicating stronger antioxidant capacity in A. dioica fruit. Higher FRAP value of 106.583 ± 5.204 ?M trolox equivalent (TE)/g dry extract (DE) was found in the methanol extract of A. dioica fruit compared to that of O. alismoides fruit (44.083 ± 7.637 ?M TE/g DE). The TPC found in the methanol extracts of A. dioica and O. alismoides fruits were 146.710 ± 2.807 mg gallic acid equivalents (GAE)/g DE and 93.860 ± 1.172 mg GAE/g DE, respectively, while the TFC was found to be 72.510 ± 8.833 mg quercetin equivalents (QE)/g DE in A. dioica fruit and 43.270 ± 5.361 mg QE/g DE in O. alismoides fruit. These fruits are good sources of nutrients and natural antioxidants and may find applications in formulation of various pharmaceutical drugs.
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