Blood samples from dogs with clinical signs compatible with ehrlichiosis were examined for infection of Ehrlichia canis using PCR, multiplex real-time PCR, and DNA sequencing analysis. Eleven of 25 samples were positive for a new strain of E. canis. This is the first molecular identification of E. canis infection in dogs from Peru.

Twenty-five isolates of Ornithobacterium rhinotracheale were examined by agar gel precipitation, immunoperoxidase assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot analysis, and a polymerase chain reaction. All of the isolates were identified as serotype A. Protein profiles of whole cell extracts were similar for all the isolates, and a polypeptide with a molecular weight of 33 kD was a major component, being present in all the isolates. In the main, proteins of 33, 42, 52, and 66 kD were recognized in immunoblots with sera from chickens naturally exposed to O. rhinotracheale. A modified polymerase chain reaction assay identified O. rhinotracheale DNA from all the isolates and tracheal swabs, producing amplicons of 784 bp, and distinguished O. rhinotracheale from bacterial agents causing similar clinical signs.

El objetivo del estudio fue determinar las biovariedades de Pasteurella multocida y Gallibacterium anatis en aves de producción con signos respiratorios. Estas bacterias fueron aisladas de muestras de secreciones y órganos de pollos de carne, gallinas de postura y patos criollos afectados, provenientes de granjas avícolas de la costa y selva del Perú. De 25 aislamientos se identificaron 13 cepas de P. multocida y 12 de G. anatis, mediante características de cultivo, morfología y pruebas bioquímicas (oxidasa, catalasa, indol, ureasa). Las cepas fueron sometidas a la prueba de fermentación en micropozos, consistente en una batería de 10 carbohidratos y un aminoácido para su tipificación. Los resultados indican que ocho cepas de P. multocida correspondieron a la biovariedad 1 y las restantes a las biovariedades 3, 4, 6, 10 y 11, en tanto que 11 cepas de G. anatis correspondieron a la biovariedad haemolytica y la otra a la biovariedad anatis.

No abstract

Forty seven clinical samples of Fowl adenovirus (FAdV) associated with Inclusion Body Hepatitis (IBH) from Peruvian broilers received between July 2006 and April 2013 were genotyped using sequencing of L1 Loop of Hexon gene. All 47 clinical samples presented macroscopic and histopathology lesions consistent with IBH, and amplified a specific fragment of Hexon gene by Polymerase Chain Reaction (PCR). A unique nucleotide sequence of 789 base pairs of Hexon gene (position 273 to 1061) was obtained in all 47 clinical samples analyzed. This sequence showed a high level of conservation in amino acid and nucleotide sequence (>99%) with a Fowl Adenovirus C serotype 4 previously identified. Sequence and phylogenetic analysis indicate no genotypic variation in Peruvian isolates. The presence of a unique genotype very closely related with genotype C1 previously reported in Peru and Ecuador (>99%), suggests the presence of FAdV C serotype 4 genotype C1 in clinical cases of IBH from Peruvian broilers.