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<p><strong>Abstract.</strong> Aquatic macrophytes are important elements of freshwater ecosystems, fulfilling a pivotal role in the ecological functions of these environments and biogeochemical cycles. Although aquatic macrophytes are beneficial, some species can hinder human activity. They can clog reservoirs and reduce water availability for human needs. Surveys of macrophytes are hindered by logistic problems, and remote sensing represents a powerful alternative, allowing comprehensive assessment and monitoring. The objectives of this study was to map temporal changes in the macrophytes using time series multispectral dataset over Tapi River, Surat. The field trip was conducted over the Tapi River on 22nd June 2018, where <i>in-situ</i> spectral response dataset were acquired using ASD Spectroradiometer. Water samples were also collected over three locations, one before entering the city (Kamrej), second at the Sarthana water treatment plant and third at the outer end (causeway). The nutrient concentration was less before entering the city (Ammonical Nitrogen 0.056<span class="thinspace"></span>mg/L and phosphate 0.0145<span class="thinspace"></span>mg/l), while higher concentration (Ammonical Nitrogen 0.448<span class="thinspace"></span>mg/l and phosphate 0.05<span class="thinspace"></span>mg/l) was observed within the city. Maps of aquatic macrophytes fractional cover were produced using Resourcesat-2/2A (LISS-III) dataset covering a period of 2012&amp;ndash;2018. Maximum extent was observed in February-March of every year. Although during monsoon, lot of agriculture run-off and nutrients will come into the river, but main flow of water will dilute its concentration. During summer, the same nutrient concentration will boost these macrophytes due to less availability of stream water. Within the area of 16<span class="thinspace"></span>km² between Kamrej and causeway, 3.35<span class="thinspace"></span>% was covered by macrophytes during March 2013. This area coverage increase to 36.41<span class="thinspace"></span>% in March 2018. Based on these maps, we discuss how remote sensing could support monitoring strategies and provide insight into spatial variability, and by identifying hotspot areas where invasive species could become a threat to ecosystem functioning.</p>