Background: Food and beverage sanitation hygiene is a prevention effort that focuses on activities or actions that are necessary to free food and drinks from hazards that can interfere with or damage health.Objective: This study aimed to identify personal hygiene, sanitation and food safety knowledge of food workers at the canteen university.Methods: This was a descriptive study with observational approach. Thirty-four canteens were recruited using total sampling. Data were analyzed using descriptive statistics with percentage.Results: Findings showed that 11 canteens (32.3%) did not meet the standard for canteen sanitation, 24 canteens (70.6%) did not meet lighting standard, 29 (85.3%) did not meet ventilation standard, 18 (52,9%) did not meet the standard of clean water, 31 (91.2%) did not meet wastewater disposal standard, 23 (67.6%) did not meet the hand washing facility standard, 25 (73.5%) did not meet standard of waste disposal conditions, 28 respondents (85.3%) had good personal hygiene, 6 respondents (14.6%) had poor personal hygiene and all food workers had good knowledge on food safety (100%).Conclusion: Personal hygiene, sanitation and food safety at the university canteen must be carried out continuously. Our findings can be used as a basis for creating healthy university canteen.

Demam Berdarah Dengue (DBD) adalah penyakit infeksi yang disebabkan oleh virus dengue dan sebagian besar penularannya berasal dari gigitan nyamuk Aedes, baik aedes aegypti ataupun aedes albopictus, Demam Berdarah Dengue (DBD) merupakan penyakit demam akut disertai dengan adanya perdarahan dalam yang memiliki kecederungan untuk menimbulkan syok atau kejang-kejang dan dapat menyebabkan kematian. Penelitian ini bertujuan untuk mengetahui adanya hubungan faktor pengetahuan, sikap, tindakan dan  ketersediaan plafon dan kawat kassa dengan kejadian DBD. Penelitian ini merupakan jenis  penelitian survey observasional analitik dengan desain cross sectional study. Penelitian  menggunakan 100 responden sebagai sampel penelitian, yang diperoleh dengan teknik  pengambilan sampel proporsional random sampling. Data dikumpulkan dengan melakukan  observasi dan wawancara menggunakan kuesioner dan selanjutnya dilakukan analisis  univariat dan bivariat. Hasil uji statistik pada tingkat signifikasi α = 0,05 diperoleh hasil  bahwa ada hubungan antara tingkat pengetahuan (ρvalue = 0.000), hubungan sikap (ρvalue = 0.013), hubungan tindakan (ρvalue = 0.000), hubungan ketersediaan kawat kassa (ρvalue = 0.000), dan hubungan keberadaan plafon (ρvalue = 0.014), Kesimpulan dari penelitian ini  ada hubungan tingkat pengetahuan, sikap, tindakan, ketersediaan kawat kassa dan  keberadaan plafon dengan kejadian DBD pada masyarakat Kecamatan Baruga. Untuk itu  diperlukan partisipasi masyarakat serta peran Puskesmas Lepo lepo dalam meningkatkan  upaya untuk pencegahan penyakit DBD pada masyarakat di Kecamatan Baruga.

Background: The main determinant of the degree of public health apart from environmental conditions is the behavior of the community. Riskesda 2007 data shows that only 38.7% of households that have practiced clean and healthy living behavior have practiced clean and healthy living behavior. In Baubau City, people still wash their hands and defecate improperly. So, they are still at risk of contracting infectious diseases. Objective: This study aims to determine the risk areas for clean and healthy living behavior in Baubau City. Methods: This type of research is an observational study with a Geographic Information System (GIS) approach. The number of respondents was 1.720 in 43 villages. Each village was represented by 40 respondents with the sampling method in each village namely simple random sampling. Results: Based on the calculation results obtained a maximum total risk index of 38 and a minimum total risk index of 9. to obtain an interval value is 7. Villages with clean and healthy living behavior Very high risk in 3 villages. High risk in 13 villages. Medium risk in 21 villages and less risky in 6 villages. Conclusion: Most of the villages in Baubau City are included in clean and healthy living behavior at medium risk.

Background: Sanitation is an effort to prevent disease by removing or regulating environmental factors related to the chain of disease transfer. Vector is an Arthropod that can cause and transmit an Infectious agent from an Infection source to vulnerable landlords.Objective: To find out the relationship between housing sanitation and the presence of vectors in densely populated neighborhoods in the Kendari sub-district of Kendari city in 2019.Methods: This was a descriptive quantitative study with a cross-sectional approach. The study was conducted between 3 April 2019 and 3 May 2019. Data were collected using Field Learning Experience Report, and analyzed using Chi-Square test.Results: Findings indicated that there was a statistically significant relationship between housing sanitation and the existence of vectors (p=.05).Conclusion:  There was a significant relationship between the physical conditions of the house, the environment with the presence of vectors in the densely populated environment of the population of Kendari District. It is suggested for the community to improve the condition of the house to meet health requirements. The Department of Health of Kendari should continuously conduct environment and housing sanitation programs.

Indonesia has various marine resources that can be used, one of which is a sea urchin (Diadema setosum), which can be used as pharmacology and food sources by using their gonad. This study aims to test the toxicity, protein content, and antioxidant activity of the ethanol extract of the sea urchin gonad. The sample tests consisted of several stages, namely: maceration performed the extraction using 96% ethanol solvent; the toxicity test used the Brine Shrimp Lethality Test method with Artemia salina Leach as the bioindicator; the protein content test used the Lowry method; anticholesterol activity test was done with ultraviolet-visible spectrophotometry (UV-Vis) method. The results showed that the ethanol extract of the sea urchin gonad had no toxic activity because the IC50 value was more than 1000 ppm, which was 1318.8639 ppm. The protein content of the ethanol extract of the sea urchin gonad was 0.0257% and had no anticholesterol potential with a value of 0.0002%. This research can be performed by conducting tests to determine aspects of reproductive biology and the anti-inflammatory effects of Diadema setosum.