No abstract

Ecat1 is a maternal effect gene that is exclusively expressed in oocytes and embryonic stem cells, and has an important role in pre-implantation development. This study was designed to investigate the expression of bovine Ecat1 gene in immature and in vitro matured oocytes as well as during early embryonic development, and also Ecat1 protein localization. Samples were obtained from slaughtered animals. RNA extractions were carried out from ovary, immature and in vitro matured oocytes and also different stages of embryonic development (2-, 4-, 8- to 16-cell stages and blastocysts). RT-PCR analysis revealed the expression of Ecat1 in ovary, oocytes and embryos. Analysis in FGENESH online tool predicted three exons and one transcription start site (TSS) in Ecat1 gene, and the 3' RACE-PCR result showed that just one splice variant was amplified. By quantitative real-time PCR technique, we showed that Ecat1 transcript increased at 8- to 16-cell-stage embryos and decreased in blastocyst stage (p < 0.05). Immunofluorescence analysis showed cytoplasmic localization of Ecat1 protein in bovine oocytes. Results demonstrated bovine Ecat1 expression at protein level and also indicated that Ecat1 has a significant higher embryonic expression at 8- to 16-cell stage. This embryonic expression is probably required for further developmental stages.

Toll-like receptors (TLRs) are known for their essential roles in promotion of innate immunity and induction of adaptive immunity through recognition of pathogen-associated molecular patterns (PAMPs) or danger associated molecular patterns (DAMPs). TLR genes are excellent models for the study of the selective pressure enforced by microorganisms on the host genome. Phylogenetic analyses have shown that interactions between pathogens and immune systems have changed during evolution. Selective pressure for maintenance of specific pathogen recognition has led to evolution of TLRs under both positive and purifying selection. However, intracellular and cell-surface TLRs have been affected differently due to their variation in conservational constraints. In this review, we summarize some of the main studies on the influence of selection on shaping the evolution of several TLR gene families in different terrestrial vertebrate species. We also describe the effect of evolution on the function of different TLRs and their specific conservations in these species and show similarities and differences in evolutionary patterns of TLR orthologs among species as well as among TLR gene families.