Grass awns are a common cause of foreign body disease in animals, but little is known about their presence in the lower urinary tract. The ultrasonographic features of grass awns in vivo and in vitro have been described in detail. The purpose of this report is to describe the clinical and sonographic features of grass awns in the urinary bladder of dogs and cats. Three male Yorkshire terriers (one of which was examined twice) and one female domestic short-haired cat were evaluated for signs of lower urinary tract disease, and an intravesicular grass awn was suspected based on ultrasound examination. The grass awn appeared ultrasonographically as a bladder stone (n = 1) or a linear hyperechoic structure (n = 4) with or without acoustic shadowing that was easy to identify due to contrast with surrounding urine. The presence of a grass awn within the urinary bladder was confirmed during exploratory surgery. In all patients, the route of entry of the grass awn was thought to have been retrograde migration from the urethral opening. The ultrasonographic appearance of grass awns in the bladder is consistent with that in other tissues.
Lymphatic endothelial cells isolated from bovine mesenteric lymphatic vessels were cultured and characterized. Lymphatic endothelial cells grew as a monolayer displaying an elongated morphology in preconfluent primary cultures. When confluent, the cells exhibited a polygonal morphology to form a "cobblestone" pattern previously described for cultured vascular endothelium. All culture lymphatic endothelial cells expressed Factor VIII-related antigen and bound Ulex europaeus I lectin. Ultrastructurally, cultured lymphatic endothelium was characterized by the presence of Weibel-Palade bodies as well as the usual cytoplasmic organelles.
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