The imperative of changes associated with digital transformation gave impetus to this study, which aims to examine the impact of digital capabilities and digital citizenship on digital transformation, as well as to identify the role of digital citizenship in the relationship between digital capabilities and digital transformation. Digital transformation is observed via change management and risk management, and two facets of digital citizenship are examined: information and data literacy and information security management. A survey was carried out, and respondents were managers employed in companies from various industries in Serbia. Considering the total sample of 224 valid questionnaires the PLS-SEM method was used to test the relationships in the proposed model. Results suggest that digital capabilities have a significant positive impact on change management, information and data literacy, and information security management. Results also confirm that digital citizenship mediates the relationship between digital capabilities and both change management and risk management.

In this paper a method for isolation and purification of the pertussis toxin from the cell culture of Bordetella pertussis was presented. Pertussis toxin production was enhanced with the optimization of the microbiological media and cultivation time. Relatively high degree of purification of 80,39% and yield of 56% was resulted by the unique combination of the protein isolation and purification methods. Using immunoelectrophoresis and high performance liquid chromatography, in comparison with pertussis toxin WHO standard, it was verified that the protein isolated is indeed pertussis toxin. The conclusion of this paper is that this unique combination of the different techniques along with the specific modifications implemented would give good results in pertussis toxin isolation and purification on laboratory level, while scaling up of this methodology could be the objective of future research. It can also be concluded that, no matter what literature data say and what opinions other authors have, all methods and working conditions should be examined and optimized for the reason of the specificity of the autochthonous species as well as the dynamic changes in this field

za proizvodnju medicinskih sredstava i jednog materijala (Polietilen NG granulat) za proizvodnju boca za infuzione rastvore. Jedna varijanta metode zasniva se na direktnom kontaktu polaznog materijala sa svinjskom krvlju, a druga na dobijanju ekstrakta polaznog materijala autoklaviranjem i kontaktu tako dobijenog ekstrakta sa svinjskom krvlju. Na osnovu dobijenih rezultata zaključeno je da svi materijali zadovoljavaju kriterijume prihvatljivosti i to u slučaju obe varijante metode ispitivanja, kao i da metoda direktnog kontakta ima veću osetljivost od metode u kojoj su korišćeni ekstrakti ovih materijala.