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SUMMARY :A survey was carried out during May, 2015 from 50 rice growing farmers to identify the farmer's practice of dose and time of P application.Based on the survey data, the average of 50 farmers P fertilizer dose (85 kg P 2 O 5 ha -1 ) was fixed as 100 % farmer's dose and majority of the farmers practice i.e. two equal splits at basal and at top dressing at early tillering stage (14 to 20 DAT) was decided as farmer practice of splitsfor conducting field experiment on rice in P accumulated soil. The field experiment was consisting of twelve treatment combinations with six levels of phosphorus (100, 75 and 50 % farmers dose and 100, 75 and 50 % RDP) and its time of application (Farmer practice of split application and basal application). As part of this investigation,The crop has given good response to application of 100 % farmers dose of P (85 kg P 2 O 5 ha -1 ) but at the same time which was found to be on par with the application of 100 % RDP (60 kg P 2 O 5 ha ) and 75 % RDP (45 kg P 2 O 5 ha -1 ). This can be inferred as saving of P fertilizer to rice from current recommended dose and farmer's dose in soils having higher initial available P. With respect to time of P application, P uptake by the grain was significantly higher in split application than the treatment receiving basal P, although this had no significant influence on the increase in grain yield of rice.How to cite this article : Archana, K., Reddy, T. Prabhakar, Anjaiah, T. and Padmaja, B. (2017). Effect of dose and time of application of phosphorus on changes in phosphorus uptake pattern and yield of rice grown on P accumulated soil. Agric. Update, 12 (TECHSEAR-4): 960-965;

Continuous use of fertilizers decreasing the biological activity of the soil. Fertile soils teem with microorganisms, which directly contribute to the biological fertility of the soil. Microorganisms are responsible for the availability of nutrients to the plants which are already exist in the soil. Hence, the present study focused on to investigate the effect of natural farming on soil microorganisms and enzymes activity of maize rhizospherein comparison with inorganic and organic farming during the autumn season of 2016-17 at Agricultural College, Jagtial. The results of the investigation revealed that the population of microbes (bacteria and fungi) at 30, 60 Days After Sowing (DAS) and harvest was unaffected by variety (Aswini) or hybrid (DHM 117). But, among the different farming methods, organic farming recorded higher bacterial (19, 29 and 22.5 x 10-7 CFU{Colony Forming Unit} g-1 dry soil) and fungi (8.25, 14.67 and 10.25 x 10-5 CFU g-1 dry soil) population at 30, 60 DAS and harvest stage respectively and significantly superior to inorganic and natural methods. The microbial population in inorganic method was inferior to that in absolute control similar to microbial population, highest urease activity was recorded in organic method at 30, 60 DAS and harvest (52.92, 68.33 and 33.25 µg NH4+ g-1 2 h-1, respectively) and it was superior to inorganic and natural farming which were at a par with each other. Significantly higher activity of dehydrogenase was observed with organic farming at 30, 60 DAS and harvest (1.65, 2.29 and 1.72 µg TPF g-1 day-1) compared to natural farming and inorganic farming. The activity of urease and dehydrogenase remained similar with DHM 117 or Aswini.

A simple, accurate, precise method was developed for the simultaneous estimation of Dapagliflozin and Saxagliptin in Tablet dosage form. Chromatogram was run through Standard BDS C8 column (50 × 4.6 mm, 5) The Mobile phase containing Potassium dihydrogen phosphate: Acetonitrile in the ratio 55:45, pH was adjusted to 3.8 with dilute orthophosphoric acid. The solution was pumped through the column at a flow rate of 1 ml/min. The column temperature was maintained at 30°C. Optimized wavelength selected was 210 nm. Retention time of Dapagliflozin and Saxagliptin were found to be 2.266 min and 2.805 min. % RSD of the Dapagliflozin and Saxagliptin were found to be 0.5 and 0.5 respectively. % Recovery was obtained as 98.98% and 98.72% for Dapagliflozin and Saxagliptin respectively. LOD, LOQ values obtained from regression equations of Dapagliflozin and Saxagliptin were 0.20, 0.60 and 0.26, 0.79 respectively. Regression equation of Dapagliflozin is y = 37377x + 89244, and y =12254x + 3122 of Saxagliptin. The retention times were decreased so that the run time also decreased. So the method developed was simple and economical that can be applied successfully for simultaneous estimation of both Dapagliflozin and Saxagliptin in bulk and combined tablet formulation.