Background: Pomegranate (punicagranatum L, Punicaceae), is an edible fruit consumed around the world. The edible part of pomegranate is rich in compounds that possess antioxidant and anti-inflammatory activities. The aim of this study is to investigate the antioxidant; anti-inflammatory and gingival wound healing effects of Punicagrantum L. seed extract oral supplementation in rabbit. Methods and Methods: Forty five male rabbits were divided into 3 groups, base line (5 rabbits) left without buccal gingival wound as( group 1),study group, 20 rabbits (group2) with buccal gingival wound treated with ethanolic extract of Punicagranatum L. seed extract and control, 20 rabbits (group 3) with buccal gingival wound only. Buccal gingival wounds were created on lower right central incisor and sutured removed after (7) days. Blood biopsies by cardiocentesis were collected at times (0, 3h, 1, 3 and 7days) for estimation of serum alkaline phosphatase activity, serum total proteins, and serum uric acid. Results: The results showed a significant increase in serum Alkaline phosphatase, total proteins and uric acid in all time intervals after buccal gingival wound, in rabbits receiving water, while their levels increased significantly only at time intervals of 3 hours and 1 day after gingival wound, in rabbits receiving pomegranate seed extraction. Conclusions: It has been concluded that oral Supplementation of pomegranate seed extract in rabbits can expedite the rate of healing of gingival wound

Background: Pomegranate (PG) fruit (punicagranatum L, Punicaceae contains many bioactive substances that play important role in the treatment of a variety of medical problems. Among PG parts, it was observed that the peel had the most potent antioxidant activity. Objective: To evaluate some serum antioxidants levels in mandibular bone injury healing in rabbits orally supplemented with pomegranate peel extract (PPE). Patients and Methods: Forty-five male rabbits (1-1.2 kg) were used and they were divided into 3 groups; the baseline group consisted of 5 rabbits without creating mandibular bone defects. 20 rabbits were used as a study group with creating mandibular bone defects and they received orally 1ml/day of PPE. Another 20 rabbits were used as a control group with creating mandibular bone defects without receiving PPE. For each rabbit of both study and control groups, a circular defect of 3 mm in diameter was made in the mid labial area of the alveolar bone of the lower right central incisor. Blood samples were taken from the baseline group and both the study and control groups at time intervals; 3hrs, 1, 3, and 7days after bone defect creation, for estimation of serum albumin (Alb), bilirubin (Bili), zinc (Zn), and magnesium (Mg) levels. Results: Serum Bili levels significantly increased 1and 3 days after bone defect creation in both study and control groups, while the levels returned back to baseline in a study group, 7 days after the operation. Serum Alb, Zn, and Mg levels showed a significant decrease in study and control groups, 1 day and 3 days after the operation, while the levels of these parameters returned back to baseline, in a study group, 7 days after bone defect. Conclusion: Supplementation of PPE can affect serum antioxidants and this was accompanied by the increase in the bone healing rate process. Keywords: Pomegranate peel, mandibular bone defect, albumin, bilirubin, divalent cations, antioxidant