Background: Oral cancer is the sixth most common malignancy that occurs in the world, with more than 330,000 deaths a year. In cancer, mutations occur in proteins, accompanied by unfolding proteins, caused by the unstable micro-environment in cells. To stabilise this condition, protein protectors called heat shock proteins (HSPs) are needed. HSPs are activated by a group of transcription factors known as heat shock factor 1 (HSF1). HSF1 is a considered target in cancer therapy. Moringa oleifera leaves are known to have anti-cancer properties because of bioactive compounds called flavonoid and isothiocyanate and are used as herbal therapy for cancer. Purpose: To investigate the potential effect of ethanolic extract of Moringa oleifera on HSF1 expression in oral cancer induced by benzo[a]pyrene. Methods: This study used 25 male Wistar rats divided into five groups consisting of the negative control group (K-), which was only given aquadest; the positive control group (K+), which was induced with benzo[a]pyrene and given aquadest; and treatment groups that were induced with benzo[a]pyrene and given Moringa oleifera leaf extract at concentrations of 3.125% (P1), 6.25% (P2), and 9.375% (P3). Examination of HSF1 expression was carried out by immunohistochemistry staining. Data were analysed using the Kruskal–Wallis test and post-hoc Tukey HSD. Results: HSF1 expression in the P1, P2, and P3 groups decreased significantly compared to the K+ group. There were no significant differences between the P1, P2, and P3 groups (p > 0.005). Conclusion: Ethanolic extract of Moringa oleifera leaves in three concentrations can decrease expression of HSF1 in oral cancer induced by benzo[a]pyrene.

Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater fish. The omega-3-PUFA in fish oil produced eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is known that EPA and DHA in essentials fatty acid (EFA) could improve BMP-2 expression. Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Purpose: To prove the increasing expression of BMP-2 after catfish (Clarias batrachus) oil application. Methods: We have used the post test only design in this research. There have been 21 Rattus novergicus as research samples, and those were divided into 3 groups, group KK as control, group KP1 was given catfish (Clarias batrachus) oil in 5% concentration, and group KP2 was given catfish (Clarias batrachus) oil in 10% concentration. Catfish (Clarias batrachus) oil were applied into the socket of dental extraction. Rat was decapulated 7 days after fish oil application and the jaw in the treated regions and control group were cut for immunohistochemistry examination to observe BMP-2 expression. Data was analyzed using one-way ANOVA test.  Result: There is significant difference increased of BMP-2 expression between control and treatment group. In samples given with 10% concentration of catfish oil had the most significant increase of BMP-2 expression. Conclusion: Catfish (Clarias batrachus) oil in 10% concentration could increase the expression of BMP-2 post dental extraction.Key words: Catfish oil, BMP-2, dental extraction.

Type 2 diabetes mellitus is a disease caused by insulin resistance which will cause blood glucose levels to increase. That factor can result in an increase of proinflammatory cytokines such as TNF- dan IL-1 which will cause the severity of the disease gets worse. Intraoral antidiabetic drugs have several side effects; therefore, it is necessary to provide probiotic supplements such as Lactobacillus casei to reduce levels of TNF- dan IL-1 so that type 2 diabetes mellitus doesn’t get worse. These bacteria can also increase levels of anti-inflammatory cytokines such as IL-10. This study aims to learn and understand more deeply about the potential of Lactobacillus casei on TNF- and IL-1 levels in type 2 diabetes mellitus. Diabetes mellitus type 2 is a chronic disease by the pancreas not being able to produce the hormone insulin in large quantities due to insulin resistance caused by impaired insulin secretion in pancreas. TNF- and IL-1 is a cytokines that plays role in the body’s defense function, if cytokines produce in excess amount it will increase the inflammatory process. Lactobacillus casei is a probiotic bacterium that can be found in yoghurt, cheese, raw meat, and other food ingredients. Lactobacillus casei has the potential to reduce TNF- dan IL-1 levels in type 2 diabetes mellitus.

Background: Dental caries occur due to a dynamic demineralization process that happens when calcium and phosphate ions are dissolved and carbohydrates are fermented by microorganisms in the mouth, leading to a decrease in saliva pH. Saliva production occurs as a response to stimuli such as tasting and chewing food. Saliva contains oral cavity bacteria, cervical gingival fluid, and leftover food, known as whole saliva. Normally, saliva pH ranges from 6.8-7.2 and can decrease if someone consumes foods and drinks containing sucrose. One way to reduce the risk of dental caries is by consuming original yogurt. Yogurt contains probiotic bacteria, namely Lactobacillus bulgaricus and Streptococcus thermophilus, which act as inhibitors of cariogenic bacteria growth. The calcium content in yogurt helps in the tooth remineralization process. Purpose: This study aims to determine the effect of consuming original yogurt on changes in saliva pH in the oral cavity. Methods: This research is pre-experimental with pretest and posttest design. The research sample consisted of 30 dental students from the Faculty of Dentistry, Institute of Health Sciences Bhakti Wiyata Kediri in 2019, 2020, 2021 and 2022, who met the sample criteria. Results: Data were analyzed using the parametric Paired T-Test hypothesis test, with a significance value of 0.000. Conclusion: Original yogurt consumption has an impact on changes on salivary pH in the oral cavity.