In the literature, the produced β-chitin samples are in powder or flake forms but there is no natural β-chitin based film. Also, the commercially available transdermal patches are produced from synthetic polymers. In this regard, we produced natural β-chitin-protein complex (CPC) film from the waste shells of Ensis spp. The obtained natural film was characterized by FTIR, TGA and SEM. Additionally, swelling, thickness, contact angle and antioxidant tests were done to learn more about the films. After production and characterization of the film, capsaicin, which is commonly used for pain relief was loaded into the film. The loading capacity was recorded as 5.79%. The kinetic models were studied in three different pH, then the results were fitted with Higuchi model with high correlation at pH 7.4. After considering all the obtained results, the capsaicin loaded CPC film may be an alternative candidate for transdermal patch instead of the synthetic ones.
Amfibiler, sucul yaşam ile karasal yaşam arasında bir köprü görevi gören Anura, Urodela ve Gymnophiona olmak üzere 3 takıma ayrılan ve dünyada 8000’den fazla türe sahip olan soğukkanlı omurgalı canlılardır. Bununla birlikte, kozmopolit olmaları, kolay kültüre edilebilmeleri, çift yaşamlı olmaları ve potansiyel klinik uygulamaları sayesinde birçok çalışmaya konu olmuştur. Çoğu hayvan türünün olgun yumurtaları, vitellin zar, jel ve zona pellusida gibi hücre dışı katmanlar veya yapılarla çevrilidir. Amfibi yumurtaları bu yapılardan bir vitellin zarf ile yapısal ve kimyasal olarak farklı birkaç jel tabakayla çevrilidir. Bu jel tabaka, amfibi yumurtalarının karakteristik bir özelliğidir. Suda çözünmeyen jelatinimsi bir matris olarak tanımlanmaktadırlar. Dışardan bakıldığında oldukça basit yapılı görünen bu jel tabakalar doğada zorlu ortam şartlarına karşı embriyoyu korumakta, beslemekte, büyümesine ve gelişmesine olanak sağlamaktadırlar. Bu denli önemli bir görev gören amfibi yumurta jeli üzerine literatürde yer alan bilgiler oldukça sınırlıdır. Bu derlemede amfibi yumurta jelinin morfolojik yapısı, içeriği, fertilizasyon üzerine olan etkisi ve immünobiyolojik aktivitesi ele alınmıştır.
Chitin is the most abundant structural biopolymer after cellulose in terrestrial ecosystems. Until now, chitin isolation in powder or granule form has been carried out from many macro living groups (Arthropoda, Crustacea, Mollusca etc.). However, studies on chitin characterization of microscopic organisms have remained limited. In this study, three dimensional (3D) chitin extraction was performed for the first time from Trachytes pauperior (Berlese), a mite species. The obtained chitin was observed by light microscopy and characterized by FT-IR, and SEM analysis. Our findings suggest that chitin, which was obtained in high purity and constitutes a large part of the organism's body structure, could be a potential source for future studies.
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