Dikerogammarus haemobaphes is a non-native amphipod in UK freshwaters. Studies have identified this species as a low-impact invader in the UK, relative to its cousin Dikero gammarus villosus. It has been suggested that regulation by symbionts (such as Microsporidia) could explain this difference in impact. The effect of parasitism on D. haemobaphes is largely unknown. This was explored herein using 2 behavioural assays measuring activity and aggregation. First, D. haemobaphes were screened histologically post-assay, identifying 2 novel viruses (D. haemo baphes bi-facies-like virus [DhbflV], D. haemobaphes bacilliform virus [DhBV]), Cucumispora ornata (Micro sporidia), Apicomplexa, and Digenea, which could alter host behaviour. DhBV infection burden increased host activity, and C. ornata infection reduced host activity. Second, native invertebrates were collected from the invasion site at Carlton Brook, UK, and tested for the presence of C. ornata. PCR screening identified that Gammarus pulex and other native invertebrates were positive for C. ornata. The host range of this parasite, and its impact on host survival, was additionally explored using D. haemobaphes, D. villosus, and G. pulex in a laboratory trial. D. haemo baphes and G. pulex became infected by C. ornata, which also lowered survival rate. D. villosus did not become infected. A PCR protocol for DhbflV was also applied to D. haemobaphes after the survival trial, associating this virus with decreased host survival. In conclusion, D. haemobaphes has a complex relationship with parasites in the UK environment. C. ornata likely regulates populations by decreasing host survival and activity, but despite this benefit, the parasite threatens susceptible native wildlife.

Biosecurity protocols designed to prevent the spread of invasive alien species (IAS) are now an essential aspect of IAS management. However, the effectiveness of various biosecurity treatments requires further exploration. Killer shrimp, Dikerogammarus villosus, a notoriously high impact and ecosystem destabilising invader, has rapidly spread across Europe, and is of concern to invade Northern America. In this study, we examine the effectiveness of three commonly used, broad-spectrum disinfectants to cause mortality of D. villosus: Virasure Aquatic, Virkon Aquatic, and Virkon S. Immersion and spray treatments of 1%, 2% and 4% disinfectant solutions were examined for applications of up to 300 secs immersion and for up to ten consecutive sprays. Furthermore, we assessed the effectiveness of steam (≥100 °C) treatments for up to 120 secs. For all disinfectants, immersion in 1% solutions caused 100% mortality at ≥120 secs. At higher concentrations, shorter immersion times caused complete mortality: 60 and 15 secs for 2% and 4% solutions, respectively. Five sprays of 2% and 4% solutions resulted in 100% mortality, for all disinfectants. Direct steam exposure was highly effective, with complete D. villosus mortality occurring at ≥10 secs. Overall, brief exposure to broad-spectrum disinfectants and direct steam could be used to limit D. villosus spread. Biological invasions of invasive alien species (IAS) are a major driver of biodiversity loss, and detrimentally affect the structuring, functioning, economic and social value of ecosystems worldwide 1,2. In an age of rapid globalisation, the anthropogenic platforms that facilitate the accidental spread of IAS are well documented and the rate of biological invasions has increased in recent years 3. Invasive alien species, synonymously termed invasive non-native species are species present outside of their native range with associated adverse impacts 4. Given the multiple dispersal pathways and array of vectors 5-8 , freshwater ecosystems in particular are considered to be especially vulnerable to the introduction and further spread of IAS 2,9,10. There is a wide range of users' that access and work in the freshwater environment, all of which can present a risk of spreading IAS from one location to another. Management options for effective and efficient control and eradication of established IAS populations are often complex, expensive, resource-intensive, and can be damaging to non-target species 10,11. Therefore, prevention of the introduction and secondary spread of IAS is the first line of defence. Biosecurity is the collectively term

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Anthropogenic stressors such as climate warming and invasive species and natural stressors such as parasites exert pressures that can interact to impact the function of ecosystems. This study investigated how these stressors interact to impact the vital ecosystem process of shredding by keystone species in temperate freshwater ecosystems. We compared metabolic rates and rates of shredding at a range of temperatures up to extreme levels, from 5 °C to 30 °C, between invasive and native amphipods that were unparasitised or parasitised by a common acanthocephalan, Echinorhynchus truttae. Shredding results were compared using the relative impact potential (RIP) metric to investigate how they impacted the scale with a numerical response. Although per capita shredding was higher for the native amphipod at all temperatures, the higher abundance of the invader led to higher relative impact scores; hence, the replacement of the native by the invasive amphipod is predicted to drive an increase in shredding. This could be interpreted as a positive effect on the ecosystem function, leading to a faster accumulation of amphipod biomass and a greater rate of fine particulate organic matter (FPOM) provisioning for the ecosystem. However, the high density of invaders compared with natives may lead to the exhaustion of the resource in sites with relatively low leaf detritus levels.