BACKGROUND: Wound healing is an essential biological process that consists of sequential steps aimed at restoring the architecture and function of damaged cells and tissues. There are empirical evidences of using pure coffee bean powder as an alternative medicine in treating various types of wounds. However, there is limited data on coffee-induced wound healing, especially migration of cells. Therefore, current study was conducted to investigate the role of coffee extract in cell migration, especially fibroblast which is important for wound healing.METHODS: Coffea canephora beans were prepared, extracted and added in the NIH3T3 cell culture in final concentration of 2.5% and 5%. Then cytotoxicity test was performed using Na,30-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate (XTT) assay. Meanwhile, cell migration test was performed with scratch assay. All results were statistically analyzed.RESULTS: The 2.5% or 5% Coffea canephora beans extract (CCBE)-treated NIH3T3 cell numbers were almost similar with the numbers of NIH3T3 cells in starvation medium merely. Meanwhile, 2.5% and 5% CCBE showed significant decrease of the widths of scratched areas compared to starvation medium merely (ANOVA with LSD Post-hoc, p=0.000). After 24 h and 48 h, the average widths of 2.5% and 5% CCBE-treated scratched areas were 235.68±22.79, 50.36±5.29, 229.95±23.01, 27.68±2.83, respectively.CONCLUSION: Since both 2.5% and 5% CCBE are potential in inducing migration of fibroblast (NIH3T3 cell) and do not induce cytotoxicity, the CCBE could be potential as an agent for wound healing.KEYWORDS: coffee, Coffea canephora, NIH3T3, migration, cytotoxicity

Background: Avulsion of permanent teeth is one of the most serious dentoalveolar injuries. Appropriate emergency management and a treatment plan are important for a good prognosis.  Therefore general dental practitioners (GDPs) must have adequate knowledge regarding the management of permanent avulsed teeth. Objective: Evaluate the knowledge base and preferred methods of general dental practitioners regarding the management of avulsed teeth. Methods: Observativational descriptive with cross-sectional was employed for sample selection. A pre-tested 13-item online questionnaire  was distributed to 98 Jakarta GDPs. The data obtained were then categorized into 3 levels which are high (≥76%), moderate (56-75%), and low (≤55%). Results: This study revealed that the majority of GDPs' knowledge is moderate. 21,4% of participants showed good knowledge, while 53,1% are moderate, and the other 24,5% lack the knowledge needed to manage avulsions. Mann-Whitney U test results showed that there was no statistically significant difference between males and females (p = 0.296) nor between the length of practice 0-10 years and >10 years (p = 0.134). Conclusions: The study findings showed that the Jakarta GDPs need to improve their knowledge about the management of permanent avulsed teeth.

This community health service aims to educate and to train St. Bellarminus Junior High School Students on the importance of dental and oral health for adolescents. The community health service program was initialized with situational analysis. Students' prior knowledge (pre-test) was assessed with 16 questions using Google form on 61 students. For the intervention, education and training was performed virtually. After the intervention, post-test was conducted to evaluate the effectiveness. Pre- and post-test questions were divided in 3 categories, which were A: general health, B: dental and oral health, C: advanced dental and oral health. Based on the pre- and post-test results, the community service program had increased the numbers of students answering correctly for each question. The percentage of students answering correctly was elevated from 57.58% (pre-test results) to 72.23% (post-test results). Meanwhile, based on knowledge category, the A and B categories were both markedly elevated after the program, suggesting the program has given positive impact for the St. Bellarminus Junior High students. Since the community health service program has given positive impact for students, hence the program should be conducted continuously.