Belonging to the Brazilian flora, the species Hancornia speciosa (Gomes), known as mangabeira, has bioactive compounds of interest, such as flavonoids, xanthones, and proanthocyanidins. The objective of this study was to determine how the supplementation of sugars in culture medium affects the osmotic potential of the medium, as well as its influence on cell growth and on the concentration of phenolic compounds. For this purpose, after 90 days of subculture, 20 mL aliquots of the cultures were added to flasks containing 20 mL of medium with different sugars (glucose, fructose, sucrose, mannitol, and sorbitol) under a 16-h photoperiod with a spectral range between 400 and 700 nm of photosynthetically active radiation (45–55 μmol m−2 s−1) in a shaker at 110 rpm. After 30 days, the pH, electrical conductivity, osmotic potential, biomass accumulation, and concentrations of phenolic compounds were evaluated. Regardless of their concentration in the medium, the sugars sorbitol and mannitol provided more unfavorable conditions for water absorption at the cellular level, reducing the water potential of the medium. Sucrose favored greater water absorption and biomass accumulation. Among the various sugar concentrations, 3% (30 g/L) sucrose or glucose improved the accumulation of fresh and dry cell weight and the production of polyphenols such as chlorogenic acid, epicatechin, rosmarinic acid, hesperidin, rutin, and quercetin. In addition, they resulted in a higher osmotic potential of the medium and larger cells than other carbon sources. Despite the differences in cell size, no culture conditions compromised cell survival.

Belonging to the Brazilian flora, the species Hancornia speciosa (Gomes), known as mangabeira, has bioactive compounds of interest, such as flavonoids, xanthones, and proanthocyanidins. The objective of this study was to determine how the supplementation of sugars in culture medium affects the osmotic potential of the medium, as well as its influence on cell growth and on the concentration of phenolic compounds. For this purpose, after 90 days of subculture, 20 ml aliquots of the cultures were added to flasks containing 20 ml of medium with different sugars (glucose, fructose, sucrose, mannitol, and sorbitol) under a 16-h photoperiod with a spectral range between 400 and 700 nm of photosynthetically active radiation (45-55 μmol m -2 s -1 ) in a shaker at 110 rpm. After 30 days, the pH, electrical conductivity, osmotic potential, biomass accumulation, and concentrations of phenolic compounds were evaluated. Regardless of their concentration in the medium, the sugars sorbitol and mannitol provided more unfavorable conditions for water absorption at the cellular level, reducing the water potential of the medium. Sucrose favored greater water absorption and biomass accumulation. Among the various sugar concentrations, 3% (30 g/L) sucrose or glucose improved the accumulation of fresh and dry cell weight and the production of polyphenols such as chlorogenic acid, epicatechin, rosmarinic acid, hesperidin, rutin, and quercetin. In addition, they resulted in a higher osmotic potential of the medium and larger cells than other carbon sources. Despite the differences in cell size, no culture conditions compromised cell survival.

No abstract

Cover plants, due to their high biomass production capacity, and plants provide the soil with several benefits such as nutrient cycling, greater water retention and storage capacity, reduced temperature, increased aggregation and microbiota, physical protection against compaction. The objective of this work was to verify the growth of the fruit species of the cerrado, Dipteryx alata Vogel, as a consortium strategy with green fertilizers, Arachis pintoi Krapov. & W.C.Greg./Callopogonium mucunoides Desv., Crotalaria juncea L, Dolichos lablab L., [Urochloa decumbens (Stapf) R.D.Webster] with nitrogen and [Urochloa decumbens (Stapf) R.D.Webster] without nitrogen (control). The experiment covered the period from 2013 to 2017, in Rio Verde, Goiás, installed in a randomized block design, with five treatments (cover plants) and four replications. The evaluations included the macro and micronutrient contents in D. alata, biomass production by cover plants, soil moisture and biometric determinations of D. alata. The use of U. decumbens with nitrogen, D. lablab and C. juncea showed better performances in maintaining soil moisture, growth and early fruiting of D. alata, making it advantageous to use these types of consortium in its establishment under conditions of the Cerrado.