The utilization of mangrove plants as traditional medicinal ingredients has long been utilized by the community for treatment of various diseases in favour of bioactive components from its secondary metabolites. Secondary metabolites from mangroves could act as an antioxidant to prevent oxidative stress. Mangrove may contain endophytic microbes in its tissues that are capable of producing secondary metabolites. In theory, endophytic microbes isolated from a plant can produce secondary metabolites similar to those of the original plants or even in relatively high numbers. In this research, two mangrove species Avicennia marina and Xylocarpus granatum from Serang, Banten province, samples have been taken to investigate the antioxidant activities of its extracts. Furthermore, isolation of endophytic microbes from both mangroves had been done. This research succeeded to isolate six fungi and three bacteria as endophytic microbes. Meanwhile, the highest extraction yield was obtained by the leaves of A. marina that is approximately 3% (w/w), whereas mangroves’ endophytic microbe highest extract yield was obtained by bacteria from fruits of A. marina 18 mg/100 mL media. For antioxidant activities, the highest activities were obtained by fungi within the stems and the roots of A. marina, also additionally fungi in the leaves of X. granatum.

The fruits or seeds of Keben (Barringtonia asiatica L. Kurz) were used traditionally for fish poisons, to curvestomach and headeach. The aim of this research was to isolate and identify bioactive compound of n-butanolfraction of active compound against Artemia salina Leach larvae. Isolation and purification of n-butanol fractionwere carried out by column chomatography (SiO2, CHCl3-MeOH) and high pressure liquid chromatography (RP,MeOH). The result of purification was than tested using BSLT methode. The test showed that, BABU-2.4 had thehighest activity with LC 50 value was 30.19 ppm. BABU-2.4 was identified with FT-IR spectrophotometer and NMR(proton and carbon) spectrophotometer as derivate of glicoside.

Isolation, purification and identification of two hydrochalcone compounds from Indonesian medicinal plant "Sirihhutan", Piper aduncum (Piperaceae) had been done. Isolation and purification of ethylacetate extract subjected to column chromatography (SiO 2 ; (i).n-hexane-ethylacetate = 10:1 ~ 1 : 1; ethylacetate (ii).n-hexaneethylacetate = 8: 1 gave two pure compounds. Based on infra red, 1D & 2D-NMR, mass spectral data and comparison chemical shift of protons and carbons, the isolated compounds are 2',6'-dihydroxy-4'-methoxy dihydrochalcone and 2',6',4-trihydroxy-4'-methoxy dihydrochalcone with free radical scavenging inhibitionvalues are 21.77 % and 90.1 % respectively.

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In addition to the bark, the leaves of the cinnamon plant are a source of natural antioxidant compounds and inhabited by endophytic fungi. The use of endophytic fungi from cinnamon leaves as a source of antioxidant compounds is not much done. This study aimed to determine the antioxidant activity of endophytic fungi were isolated from young and old leaves of cinnamon plants. Isolation of endophytic fungi was done by planting leaf tissue on Potato Dextrose Agar media after surface sterilization with 70% ethanol for 1 minute, 5.3% sodium hypochlorite for 5 minutes, and 70% ethanol for 30 seconds, respectively. Antioxidant activity is carried out based on the α, α-diphenyl-β-picrylhydrazyl free radical scavenging method. The ethyl acetate extract of endophytic fungi with the highest antioxidant activity on each type of leaf was carried out by profiling chemical compounds using GCMS. The extracts of eight endophytic fungal isolates obtained had antioxidant activity. Cb.Dm3 and Cb.Dt2 endophytic fungal extracts had the highest antioxidant activity with IC50 values of 62.80 and 8.11 µg.ml−1, respectively. Profiling chemical compounds showed that both isolates contained antioxidant compounds with Cb.Dt2 isolate containing cinnamaldehyde. Cb.Dt2 endophytic fungal extract has the potential as a potential source of natural antioxidant compounds.