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In this study, N,N'-(dodecane-1,12-diyl)bis(2,4-dichlorobenzamide) and N,N'-(dodecane-1,12-diyl)bis(4-bromobenzamide) as new bis-benzamides were synthesized by reaction of 1,12-diaminododecane and two different acyl chloride compounds in 88% and 92% yield, respectively. Their molecular structures were characterized using 1H NMR, 13C NMR and FT-IR techniques. Antibacterial activities of the synthesized compounds were screened against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 strains. Moreover, photophysical properties of the products in CH 2 Cl 2 and CHCl 3 were investigated using UV-vis spectroscopy. The compound 3a exhibited positive solvatochromism about 31 nm by increasing of solvent polarity.