Purpose: To determine whether rocuronium would provide safe, short-term immobilization in Podocnemis expansa. Methods: Twenty P. expansa, weighing on average 1.59 ± 0.28 kg, were subjected to two protocols: G1 0.25 mg/kg IM of rocuronium and 0.07 mg/kg IM of neostigmine, while G2 received 0.50 mg/kg IM of rocuronium and 0.07 mg/kg IM of neostigmine. The drugs were applied, respectively, in the left and right thoracic members. Assessments were made of the anesthetic parameters of respiratory frequency, heartbeat, righting reflex, cloacal relaxation, palpebral and pupilar reflexes, easy handling, muscle relaxation, locomotion, response to pain stimuli in the right thoracic members, pelvic members and tail, ambient humidity and temperature. Results: They were not found statistical differences between the dosages for the majority of the assessments. G1 was as efficient as G2. A consistent neuromuscular blockade effect was recorded 12 ± 4.21 minutes in G1 and G2. All the animals were recovered in 150 minutes. Conclusions: Administration of rocuronium at dose of 0.25 to 0.5 mg/kg IM is a safe and effective adjunct to clinical proceedings or pre-anesthetics in P. expansa. Because rocuronium does not provide any analgesic or sedative effects, the duration of neuromuscular blockade without anesthesia should be minimized to avoid undue stress. RESUMO Objetivo: Determinar se o rocurônio promove imobilização segura e de curta duração em Podocnemis expansa. Métodos: Vinte P. expansa com média de peso 1,59 ± 0,28 kg, foram submetidas a dois protocolos: G1 recebeu rocurônio 0,25 mg/kg IM e neostigmina 0,07 mg/kg IM enquanto G2 rocurônio 0,50 mg/kg IM e neostigmina 0,07 mg/kg IM, aplicados no membro torácico esquerdo e direito, respectivamente. Observaram-se os parâmetros anestésicos: freqüência respiratória e cardíaca, reflexo de endireitamento, relaxamento do esfíncter da cloaca, reflexo palpebral e pupilar, facilidade de manipulação, relaxamento muscular, locomoção, resposta aos estímulos dolorosos no membro torácico direito, nos membros pelvinos e na cauda, temperatura e umidade ambiental. Resultados: Não foram encontradas diferenças estatísticas entre as doses para a maioria dos parâmetros e o G1 foi tão eficiente quanto o G2. Um bloqueio neuromuscular consistente foi observado aos 12 ± 4,21 minutos tanto no G1 como no G2. A recuperação de todos os animais ocorreu em até 150 minutos. Conclusões: Administração de rocurônio nas doses 0,25 e 0,50 mg/kg IM é segura e efetiva para os procedimentos clínicos ou pré-anestésicos em P. expansa. Como o rocurônio não produz efeitos sedativos ou analgésicos, a duração do bloqueio neuromuscular sem anestesia deverá ser minimizado para evitar estresse.

The objective of this research was to determine a protocol for effective and safe pharmacological restraint (Phrynops geoffroanusi), with average weight of 1.9 ± 0.77 kg, clinically healthy, 02 males and 18 females, capturing in Uberabinha river in the city of Uberlândia - MG under license M ° 032/2006 IBAMA-RAN, were housed in a tank with running water and water heaters with an average temperature of 27 ° C. the turtles were divided into two groups of 10 animals: G1 - midazolam (2.0 mg / kg IM) and propofol (5.0 mg / kg IV) and G2 - midazolam (2.0 mg / kg IM) and propofol 2.5 mg / kg IV). Midazolam was administered in the left forelimb and propofol was injected directly in the cervical vertebral sinus. There was the anesthetic parameters of movement, muscular relaxation, in response to stimuli forelimbs and tail, ease of handling and heart rate, at 0 and 5, 10, 20, 30, 45, 60, 90, 120, 150 and 180 minutes after the injections. There was no statistically significant between two groups, so this protocol is not indicated for surgical procedures in Phrynops geoffroanus.

Eggshell evaluation may serve as an indicator of the effect of substances released in the environment, which may change eggshell shape, size, structure, and/or chemical composition. Additionally, exposure may interfere with hatching rates in contaminated eggs. The objective of this study was to better understand how exposure to the insecticide methyl parathion interferes with chemical changes in eggshells of Podocnemis expansa throughout their artificial incubation, as well as with egg hatchability. A total of 343 P. expansa eggs were collected in a natural reproduction area for the species. These eggs were transferred to and artificially incubated in the Wild Animal Teaching and Research Laboratory at Universidade Federal de Uberlândia. On the first day of artificial incubation, 0, 35, 350, and 3500 ppb of methyl parathion were incorporated to the substrate. Eggs were collected every three days for chemical analysis of eggshells. Hatchability was evaluated as the number of hatchlings in each treatment, for the eggs that were not used in the chemical analysis. Student's T-test was used for data on eggshell chemical composition, and the Binomial Test for Two Proportions was used in the hatchability analysis, at a 5% significance level. It was observed that the incorporation of methyl parathion to the substrate on the first day of artificial incubation of P. expansa eggs reduced the levels of total fat in the shells throughout their incubation, besides reducing egg hatchability.

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1. Docente. 2. Mestrandos. 3. Doutoranda. ResumoA Brucelose é uma doença infecto-contagiosa provocada por bactérias do gênero Brucella, de distribuição mundial, infecta diversas espécies de animais domésticos e até mesmo os humanos. Dentre as espécies animais em que a doença já foi diagnosticada incluem bovinos, caprinos, suínos, cães, ovinos, eqüinos, bubalinos e ratos do deserto. Poucos trabalhos na literatura abordam a doença na espécie eqüina, dificultando valores sobre a distribuição da doença a nível nacional e mundial. O presente trabalho teve como objetivo avaliar a prevalência da doença em eqüinos que prestavam serviço de tração em áreas rurais do município de Uberlândia-MG e esclarecer os mecanismos epidemiológicos envolvidos na cadeia de transmissão na região. Foram utilizados testes sorológicos, e, um questionário sócio-epidemiológico foi respondido por cada proprietário dos animais amostrados após a coleta do