The antifungal activity of ethanolic extracts of Rosmarinus officinalis and Thymus vulgaris were tested against strains of Aspergillus flavus and A. ochraceus, since these two species are common contaminants of cereals and grains and are able to produce and accumulate mycotoxins. The methodology used is based on measuring the inhibition halos produced by discs impregnated with the extracts and establishing their Minimum Inhibitory Concentration (MIC) as well as the Minimum Fungicide Concentration (MFC). The results obtained suggest that the assayed extracts affect the proper development of A. flavus and A. ochraceus; leading to a lower MIC (1200 ppm) and MFC (2400 ppm) for T. vulgaris extract against A. ochraceus than against A. flavus. The results show, that the extracts of Rosmarinus officinalis and Thymus vulgaris used at low concentrations could have significant potential for the biological control of fungi in foodstuffs.
Eighty dermatophyte strains belonging to the genera Trichophyton, Microsporum, Epidermophyton and Chrysosporium were screened for their ability to produce extracellular enzymes using a semiquantitative method. The results obtained vary significantly when the studied genera are observed separately, though the enzyme beta-galactosidase was detected in none of the four studied genera.
The characteristics of 23 strains of Trichophyton mentagrophytes isolated from clinical samples, were studied. Sabouraud agar was the most adequate medium to inhance at their utmost the different taxonomical features. Variability of enzymatic reactions in strains of T. mentagrophytes was observed.
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