The aim of this study was to evaluate pain intensity and wound healing of the post-extraction sites after the use of Platelet-Rich Fibrin (PRF). 240 patients which needed difficult or multiple extractions were included in the study. The extractions were realized under local anesthesia, with piezotome and pliers. The patients were divided in two groups, the first group of 120 patients which benefited the application of PRF in the post-extraction alveoli, and the second group of 120 patients represented the control group (without application of PRF). A questionnaire was realized after a VAS analog scale and was used to quantify the post-intervention pain intensity in the postoperative four days. The assessment of the post-extraction sites healing period was performed clinically. According to the obtained results, the healing period of the post-extraction sites was shorter in the patients of first group (PRF), compared to the sites of control group patients. No postextractional healing complications occurred in the patients of first group/PRF. The study demonstrated the benefits of PRF insertion in post-intervention healing.

Nowadays, eating habits and lifestyles are moving towards a diet high in sugars and carbohydrates, which is extremely harmful to both oral integrity and systemic health. The aim of the study was to identify the variations between the bacterial plaque formed in carious lesions, infected root canals and prosthetic components, in comparison with commensal oral microflora. The study was carried out on a group of 21 patients aged between 7 and 84 years, 13 women and 8 men, with various oral diseases, which were diagnosed during the specialized examination. The preparation of the medical record, the signing of the informed consent, the anamnesis and the consultation were carried out in the private dental office where the patients presented for a personalized treatment plan, and the examination of the pathological products and the interpretation of the results obtained in the Microbiology Discipline, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania. The study carried out on the preparations taken from patients with carious lesions reveals the presence of numerous G+ cocci arranged in short chains (23%), frequent G+ cocci in diplo, thin G+ bacilli with rounded ends and G+ cocci in diplo, arranged in small piles (15 %), and values lower than 8% are represented by G+ in diplo cocci, lanceolate, rare G+ in diplo cocci, very rare fusiform bacilli and dentinal fragments. The study carried out on the preparations taken from the endodontic space showed a remarkable percentage of epithelial cells and leukocytes (28%), rare fusiform bacilli, very small fragments of epithelial tissue, thin G+ bacilli with rounded ends, G+ cocci in diplo and in small piles and buns arranged in short chains (5-6%). The study carried out on the preparations taken from the level of prosthetic components, recorded a relatively high number of epithelial cells (19%), G+ cocci arranged in diplo, lanceolate and in small piles (13%), thin G+ bacilli, with rounded ends (10 %), cocci in short chains (10%) and rare polymorphonuclear leukocytes (10%), while at the opposite pole, squamous epithelial cells, fusobacteria and pleomorphic forms (3%) are found.

Currently, the concept of oro-dental prophylaxis and prevention is becoming more and more publicized among the population. These notions promote both the prevention of the development of dento-periodontal and oral structures diseases, diagnosing the diseases of the oral cavity and stopping their evolution, as well as the prevention of complications in the oro-maxillo-facial sphere. The purpose of this paper was to perform a comparative experimental study between the commensal oral flora and the composition of the biofilm after tooth brushing with Sensodyne Rapid Relief toothpaste and Lacalut toothpaste with hydroxyapatite. The study group included a number of 15 patients, of which 9 men and 6 women, aged between 20 and 71 years. The collection of biological preparations took place in the dental office, where the patients presented themselves for an individualized treatment plan, and the analysis and interpretation of the results were carried out in the Microbiology Discipline, “Carol Davila” University of Medicine and Pharmcy, Bucharest, Romania. According to the results of our study, following dental brushing with Lacalut toothpaste with hydroxyapatite, its effectiveness was demonstrated on species such as G+ lanceolate cocci, curved G+ bacilli, G- spindle bacilli or G- filamentous formations, while the effect antibacterial for G+ shells in diplo and short chains was not as expected. Sensodyne Rapid Relief Toothpaste has been shown to have a positive effect on species of G+ lanceolate cocci, G- fusiform bacilli, round-headed G+ bacilli, curved G+ bacilli or G- filamentous formations. On the other hand, on G+ cocci in diplo and short chains and epithelial cells it did not show the desired efficiency, these being found in the highest percentage. This study once again shows the importance of microscopic examination in assessing the effect of some toothpastes on the weight of the oral flora.