Whole saliva from all patients with periodontal disease had higher sIgA and lower peroxidase content. Smokers with periodontitis had higher sIgA than smokers without periodontitis. Smokers with and without periodontitis had higher collagenase activity than non-smokers (with and without periodontitis).

Lactate dehydrogenase may distinguish localized and generalized aggressive periodontitis. Alkaline phosphatase increases from moderate to severe states in both types of periodontitis. Aspartate aminotransferase and neutrophil elastase only increase with strong evidence of periodontal destruction.

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Background:Periodontitis is an inflammatory disease characterized by connective tissue breakdown and alveolar bone resorption.Objective:The aim of this study was to make a quantitative evaluation of the gingival tissue components in biopsies from patients with different clinical states of periodontal disease and to determine the relationship between the presence of interleukin-8 and the tissue destruction.Materials and Methods:The study group consisted of 33 biopsies from adult subjects. A total of 25 periodontal patients were sub-classified on the basis of the clinical and radiographic criteria in mild, moderate and advanced periodontitis. Gingival samples were obtained from patients in the course of basic periodontal surgeries as a muco-periostic collate. Data were analyzed by the SPSS system.Results:Collagen fibres decreased as non-specific chronic states increased. A lymphocyte inflammatory infiltrate changed to a lymphoplasmocytary form. Reactivity to interleukin-8 was detected with the severity of the histopathologic diagnoses; however there was no association with the clinical diagnoses.Conclusions:Histopathology and histomorphometry of gingival tissue changed with the degree of inflammation. No evidence of interleukin-8 as a biomarker for clinical diagnoses of periodontitis was obtained.

To quantify biomarkers of inflammation and tissue destruction in gingivo crevicular fluid to contribute to the diagnosis of the severity of the gingivo-periodontal disease.115 adults with gingival-periodontal disease were included. Diagnosis was determined through clinical and radiographic criteria. Samples were obtained from six to eight sites per patient. Data were analyzed by an SPSS system using ANOVA One Way and Tukey tests.Volume raised in gingivitis, moderate and severe periodontitis Total proteins and hydroxyproline increased in moderate and severe periodontitis. Electrophoresis evidenced protein bands that increased with the severity of periodontitis. Some were similar to serum proteins and an additional band of less than 14 kDa was present in all the periodontitis samples. Collagenase bands evidenced differences between healthy and diseased subjects. Calcium determinations showed no statistically significant differences among groups.Levels of gingivo crevicular fluid biomarkers specific for inflammation and collagen degradation correlate with the clinical diagnosis in patients with gingivitis and chronic periodontitis.