BackgroundMeloxicam (MLX) is a nonsteroidal anti-inflammatory drug used in the relief of postoperative pain for human and veterinary medicine. This study was designed to investigate the effect of surgery on the plasma disposition of MLX in dogs undergoing ovariohysterectomy following a single intravenous injection at a dose of 0.2 mg/kg bodyweight. Eight crossbred bitches were used in the study. A two-phase experimental design with a 10-day washout period was used. Pre-operative MLX was administered intravenously to 8 bitches about 10 days before surgery (Phase I, control) at a dose of 0.2 mg/kg bodyweight and peri-operative MLX was administered intravenously after anaesthesia and 15 min before the start of surgery (Phase II). Blood samples were collected from all animals at various times between 1 and 96 h after the drug administrations in both phases. The drug concentrations were analysed using high performance liquid chromatography.ResultsThe volume of plasma MLX distribution at steady-state (Vdss) of the control group (Vdss: 263.0 ml/kg) was significantly greater (P < 0.05) compared to that of the surgery group (Vdss: 149.3 ml/kg). The AUC values were higher (29.5 vs. 23.0 μg.h2/ml) and the CL values were lower (7.7 vs. 10.5 ml.h/kg) in the surgery group compared to the control group, respectively, but differences were not significant.ConclusionsThe results of the present study indicated that surgery could alter the plasma disposition of MLX and thus the drug efficacy and side effects such as gastrointestinal ulceration, unusual bleeding and loss of kidney function/failure when repeated doses are used.

The effect of subclinical mastitis on serum estradiol and tumor necrosis factor alpha (TNF-α) levels during oestrus and subsequent fertility was investigated in dairy cows. 40 cows were divided into two groups as healthy control (n=20) and subclinical mastitis (n=20), according to the results of California Mastitis Test (CMT) and bacteriological isolation and identification. Cows were synchronised with a standart 7 day Ovsynch protocol. Following prostaglandin F2alpha (PGF2α) administration, cows were examined with trans-rectal ultrasonography at 24, 36 and 48 th h, dominant follicle diameters were recorded and blood samples were collected. Sixteen h after the second gonadotrophin-releasing hormone (GnRH) administration, cows were inseminated and a final examination of ovaries were performed and dominant follicle diameters were recorded. estradiol and TNF-α concentrations were analysed with ELISA in serum samples. No significant differences were found between the follicular diameters and growth patterns (P>0.05) of the two groups while estradiol concentations were significantly higher in the subclinical mastitis group than the control group at 24 and 48 h after PGF2α injection (P=0.017 and P=0.036 respectively). Also TNF-α levels were significantly higher in cows with subclinical mastitis than the control group (P=0.03). Positive correlations were observed between estradiol and TNF-α levels, in both groups (Control Group: R=0.512, P=0.021; Subclinical Mastitis Group: R=0.826, P<0.001). Overall pregnancy rate was higher in the control group (40%) than the subclinical mastitis (25%) group however the difference was not statistically significant (P>0.05). In conclusion estradiol and TNF-α concentrations were found higher in cows with subclinical mastitis during estrus and this data may be due to a luteal insufficiency during the initiation of synchronization, however further studies are required. Özet Süt ineklerinde subklinik mastitislerin serum östradiol ve tümör nekrozis faktör alfa (TNF-α) düzeyleri ve fertilite üzerine etkileri araştırıldı. Kaliforniya Mastitis Test (CMT) ve bakteriyolojik izolasyon identifikasyon sonuçlarına göre 40 inek sağlıklı kontrol (n=20) ve subklinik mastitisli (n=20) olmak üzere iki gruba ayrıldı. İnekler standart bir 7 günlük Ovsynch protokolü ile senkronize edildi. Prostaglandin F2 alfa (PGF2α) uygulamasını takiben inekler 24, 36 ve 48. saatlerde transrektal ultrasonografi ile muayene edildi, dominant follikül çapları kaydedildi ve kan örnekleri toplandı. İkinci gonadotropin salgılatıcı hormon (GnRH) uygulamasını takiben 16. saatte inekler tohumlandı, son bir ovaryum muayenesi yapıldı ve dominant follikül çapları kaydedildi. Serum örneklerinde östradiol ve TNF-α düzeyleri ELISA ile belirlendi. Folikül çapları ve folliküler gelişim özelliği yönünden iki grup arasında önemli bir fark görülmezken (P>0.05), serum östradiol konsantrasyonları subklinik mastitis grubunda kontrol grubuna göre PGF2α enjeksiyonunu takiben 24 ve 48. saatlerde belirgin ölçüde yüksek bulundu (sırasıyla, P=0.017 ...

A four-year-old Anatolian Shepherd bitch was brought to our clinic with an intermittent purulent-hemorrhagic vaginal discharge complaint with no general symptoms for six months following the last parturition. There were no signs of mass or lesion on the vaginal wall during physical examination. Anechogenic uterine content, hyperechoic structures freely floating in the lumen, and suspicious fetal tissue remnants were detected by ultrasonography in only the left uterine horn. Hematological parameters revealed the chronic uterine infection. The unilateral ovariohysterectomy operation was performed for the more affected left horn to maintain the patient's fertility. Two fetal bone remnants were detected in the reddish infectious uterine content at the postsurgical uterine incision. Throughout the postoperative five months, vaginal discharge or any general signs of complication were not observed. We thought that the last fetus might have undergone uterine retention and autolysis in the following weeks, which caused the chronic uterine infection. Obstetrical controls are well-advised in all parturitions to reveal any fetal remnant cases/complications and to have a normal postpartum process.

The study aimed to observe the effects of two forage sorghum hybrids and different nitrogenous (N) fertilizer rates. The current study was set up in randomized complete block (RCB) as a split-plot design with triplicate at the Field Crops Department experimental area, University of Ankara in the 2022 summer season. This experiment included two treatments which had forage sorghum hybrids (Hay day and Super-graze) and nitrogen fertilization rates in the form of 18% ammonium sulfate [control group with no nitrogen (N0), 120 kg ha-1 N (N1), 180 kg ha-1 N (N2)]. N fertilizing rates positively influenced in agronomic profiles, yield components and nutritive profiles. The plant height (197.24-221.72 cm), green herbage yield (GHY) (60.42-70.89 ton ha-1), dry matter (DM) yield (17.77-23.98 ton ha-1), crude protein (CP) yield (0.97-1.90 ton ha-1), metabolic energy (ME) yield (33070.17-51840.85 Mcal ha-1), net energy production (NEp) yield (21164.91-33178.15 Mcal ha-1) increased with applied N rates. The maximum of DM (33.83%), CP (7.90%), TDN (58.63%), ME (2.16 Mcal kg-1 DM), NEp (1.80 Mcal kg-1 DM), and the minimum of ADF (33.09%), NDF (53.17%), ADL (5.14%) were obtained in N2 rates. In light of the results; “Hay-day” had more GHY. “Sugar-graze” had more DM yield and it was determined more digestible forage. It was inferred that the best yield components and nutritive profiles were obtained in N2 rate.

A four-year-old pluriparous sheep was brought to our clinic with the complaints of mild anorexia and a wound on the ventral abdominal area, with part of a dead lamb protruding from this lesion. Clinical examination revealed that there was a fetal retention into the ruptured gravid uterine horn that was trapped within a ventral hernia. At herniorrhaphy after removing the dead fetus, strong connections between the uterine and abdominal wall and chronic scars/necrosed tissues were detected in the wound edges, which revealed the long time lapse between the unnoticed herniation-tearing and surgery. Although many ventral hernia cases have been reported in ovine pregnancies, the high maternal resilience in this ruptured hysterocele case, which had a two-week-old history at minimum, is clinically remarkable.