Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a welladapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F 1 to ICF 3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC 2 F 1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF 3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF 3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.

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Background: Green gram [Vigna radiata (L.) Wilczek] is an ancient and well known pulse crop of Asian countries. Specific traits should be considered for high yield in green gram as selection criteria in future breeding program.Methods: The investigation material comprised of 128 F6 RIL (Recombinant Inbred LINE) population sown at college farm, Professor Jayashankar Telangana State Agricultural University during Rabi-2019-20. The yield and yield attributes were recorded to conduct genetic variability, heritability broad sense (h2), genetic advance (GA) and genetic advance as % of mean (GAM).Result: Analysis of variance revealed significant differences among the RILs, indicating the presence of genetic variability for almost all the traits studied. High PCV and GCV estimates was noticed for number of pods per plant, seed yield per plant, number of cluster per plant and number of pods per cluster. High heritability along with high genetic advance as per cent of mean was observed for number of pods per plant, seed yield per plant, number of cluster per plant, number of pods per cluster, number of branches per plant, number of seeds per pod and plant height indicating the role of additive genes in governing the inheritance of these traits and could be improved through selection. The traits viz. seed yield per plant, number of pods per plant, number of clusters per plant and number of pods per cluster had recorded high PCV, GCV, high heritability along with high genetic advance as per cent of mean indicated these traits were less influenced by environment and possess high genetic variability. Hence these RILs would be suitable for green gram breeding programmes to develop improved varieties. The present findings of the RIL population will be useful for development of high seed yielding variety in green gram.

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