Background Prucalopride drug estimation was reported previously by UV spectroscopy methods and few HPLC methods with retention time 4.73 min as per literature survey. Aim of study A new analytical RP- HPLC method development and validation for bulk and pharmaceutical dosage forms of Prucalopride drug with less retention time than reported methods.Methodology The separation was carried out by using RP-HPLC with the mixture of 0.1 orthophosphoric acid methanol in ratio of 3070 vv as mobile phase. The mobile phase at a flow rate of 1 mLmin was pumped and UV detection was found at 225 nm.Results The selected chromatographic conditions gave retention time at 1.5 min for Prucalopride. Conclusion This developed method was validated for precision accuracy linearity robustness as per International Conference of Harmonization ICH guidelines.

Background Rofumilast drug estimation was done earlier by spectroscopic methods. Only few chromato graphic studies were reported. Objective Development and validation of RP-HPLC method for the drug Roflumilast by using various chromatographic parameters. Methodology RP-HPLC method was developed with 1 mlmin as flow rate and 10 microl was set as volume of injection for about 15 minutes of run time. Various parameters like accuracy linearity precision were validated for the method as per ICH guidelines.Results UV detection for maximum wavelength at 250 nm with mobile phase of 3070 ratio of Trifluroacetic acid pH 3.2 Acetonitrile showed the retention time of 2.317 min. Conclusion The proposed RP- HPLC method was found to be rapid simple precise accurate and economic forestimation of roflumilast in bulk and pharmaceutical formulations.

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