No abstract

No abstract

No abstract

In this study, we developed Lava-Dermabiotics HDB1234 using Lactobacillus plantarum HDB1234 and lava seawater fermentation technology to enhance skin barrier. The use of lava seawater increased the purity and concentration of nucleotides in Lava-Dermabiotics HDB1234 by using nanodrop 2000 spectrophotometer. Also, when 5%(v/v) Lava-Dermabiotics HDB1234 was treated, cytotoxicity on normal human epidermal keratinocytes (NHEKs) and human fibroblast cells was not observed by using MTT assay. The mRNA expressions of claudin-1 (CLDN-1), serine palmitoyltransferase (SPT), and filaggrin (FLG) on Lava-Dermabiotics HDB1234 treated cells were dose-dependent higher than those of non-treated cells. Especially, the protein expression of FLG was also confirmed by using western blotting and immunocytochemistry staining. These results indicated that Lava-Dermabiotics HDB1234 could be used as a potential cosmetic ingredient for skin barrier enhancement through CLDN-1, SPT, and FLG expression related to the formation of skin barrier.

Fermentation has recently re-emerged as an approach for improved functionality of food products in addition to the traditional roles such as shelf life, taste, and texture. Here, we report dynamic changes in the metabolite profiles of Achyranthes japonica Nakai by Lactobacillus plantarum fermentation, primarily, the significant increases in representative functional ingredients, 20-hydroxyecdysone and 25S-inokosterone. Additionally, untargeted metabolite profiling showed 58% of metabolites underwent significant alteration. The most dynamic change was observed in cellobiose, which showed a 56-fold increase. Others were sugar alcohols and amino acids, while lyxitol and erythritol that were among the most dynamically down-regulated.