Symptoms suggestive of GER are not rare in childhood, yet only a fraction of children with symptoms are treated with over-the-counter antacids or histamine2 antagonists. Prospective longitudinal data are needed to determine which children with symptoms of GER actually have GER disease and are at risk of developing complications.

The citrus whitefly, Dialeurodes citri (Ashmead), is one of the three economically important whitefly species that infest citrus plants around the world; however, limited genetic research has been focused on D. citri, partly because of lack of genomic resources. In this study, we performed de novo assembly of a transcriptome using Illumina paired-end sequencing technology (Illumina Inc., San Diego, CA, USA). In total, 36,766 unigenes with a mean length of 497 bp were identified. Of these unigenes, we identified 17,788 matched known proteins in the National Center for Biotechnology Information database, as determined by Blast search, with 5731, 4850 and 14,441 unigenes assigned to clusters of orthologous groups (COG), gene ontology (GO), and SwissProt, respectively. In total, 7507 unigenes were assigned to 308 known pathways. In-depth analysis of the data showed that 117 unigenes were identified as potentially involved in the detoxification of xenobiotics and 67 heat shock protein (Hsp) genes were associated with environmental stress. In addition, these enzymes were searched against the GO and COG database, and the results showed that the three major detoxification enzymes and Hsps were classified into 18 and 3, 6, and 8 annotations, respectively. In addition, 149 simple sequence repeats were detected. The results facilitate the investigation of molecular resistance mechanisms to insecticides and environmental stress, and contribute to molecular marker development. The findings greatly improve our genetic understanding of D. citri, and lay the foundation for future functional genomics studies on this species.

A series of mono-, di-, tri-, and tetrasubstituted 9-phenanthrene amino alcohols has been prepd in which each compd bears at least one CF3 group. A number of these compds, triand tetrasubstituted with a combination of CF3 and Cl groups, are the most active, nontoxic amino alcohols to emerge from the vast primary screen {Plasmodium berghei, mouse) of the Army's Research Program on Malaria. The most effective member of the series, 6,7-dichloro-2,4-bis(trifluoromethyl)-a-(di-n-propylaminomethyl)-9-phenanthrenemethanoTHCl (159), is 100% curative at 5 mg/kg and active at concentrations as low as 1.25 mg/kg. Antimalarial enhancement of 9-phenanthrenemethanols by introduction of CF3 groups or a combination of CF3 and halogen was described earlier.1 In an effort to approach the optimal substitution pattern for this series we have synthesized the compds included in Table I.Chemistry. The preparative routes were essentially those described in paper 1,1 Details have been tabulated in the Experimental Section.

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