Bacterial leaf damage or blight brought by bacteria Xanthomonas oryzae pv. oryzae (X. oryzae pv. oryzae) is considered as an extremely serious disease of rice worldwide, including Indonesia. Induced resistance using chemical and biological agents was considered as a method to control the disease. The objectives of this research were to analyze of endophytic bacteria (Lysinibacillus sphaericus/L.sphaericus) and salicylic acid as the inducers of rice resistance against X. oryzae pv. oryzae. This study used three-factorial incompletely randomized designs to study the effect of endophytic bacteria and salicylic acid on three varieties of rice. The results of the study showed that endophytic bacteria and salicylic acid induced rice resistance to X. oryzae pv. oryzae. This endophytic bacterium was also able to increase the plant height (AUPHGC), the number of tillers (AUNTGC), and the number of productive tillers. The application of salicylic acid at the concentrations of 10 mM was able to suppress the BLB disease and increased the number of tillers and 1000-grain weight. The result of study showed that the endophytic bacteria and salicylic acid could induce resistance of rice varities against BLB disease and increased the number of productive tillers.
The use of salicylic acid as resistance inducers agents in several plants species was well known. Salicylic acid has been believed to play an important role in inducing resistance against some pathogen. This research was aimed to study the role and effectiveness of salicylic acid as inducers for the resistance of some rice variety against bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. The experiment was arranged in completely randomized factorial design with three factors i.e. rice variety, salicylic acid, and pathotypes, each treatment was replicated three times. The results showed that salicylic acid could prolong the latent period of X. oryzae pv. oryzae pathotypes IV and VIII in paddy rice varieties Conde and Ciherang, induce the resistance of rice varieties as well as reducing the rate of infection of the two mentioned pathotypes. Salicylic acid was also able to induce the resistance of Conde and Ciherang varieties in order to suppress area under disease progress curve (AUDPC) of X. oryzae pv. oryzae pathotypes IV and VIII.
The growth of mustard plants is influenced by the availability of nutrients through the application of fertilizers. Manure and sea mud are one type of biological fertilizer. The study aims to determine the optimal dose of sea mud and manure for the growth and production of mustard plants. The study design was a randomized group design consisting of 2 factors and repeated 3 times. The first of Sea Mud treatment (L) consists of levels, namely: L0 = Control; L1=500 g, L2= 750 g. L3 1000 g. The second factor is that the dose of manure consists of levels, namely: P0=without manure; P1=120 g, P2=160 g: and P3 = 200g. The results showed that L3 treatment was able to increase plant height, while the number of leaves was best in L2 treatment. This treatment was also able to increase the number of leaves, fresh weight and dry weight but between L2 and L3 treatments did not differ markedly. P3 manure treatment is able to increase the best number of leaves, while fresh weight is best P2 treatment. The interaction between treatments only occurred in plant height and leaf area parameters at 17 HST observations.
Sago is a staple plant of the people of Maluku and Papua, which have many benefits and advantages to continue to be developed. Endophytic bacteria are bacteria that live in plant tissues and colonize the intercellular and vascular systems. This study aims to obtain endophytic bacterial isolates from parts of the roots, stems, and leaves of sago plants which have the potential as agents for plant growth promoting bacteria. Endophytic bacteria are isolated from the roots, stems, and leaves. Based on the results of isolation, 21 isolates of endophytic bacteria were found. Then, the selection is done by hypersensitivity test and gram reaction test. The results of the selection obtained 20 isolates of endophytic bacteria that were not pathogenic. After that, it was followed by plant growth promoting test for endophytic bacterial isolates. The test results were obtained three isolates potentially as plant growth promoter that is STA1, STA6, and STA11. Keywords: endophytic bacteria, plant growth promoting, sago ABSTRAK Tanaman sagu merupakan tanaman pokok masyarakat Maluku dan Papua, yang memiliki banyak manfaat dan keunggulan untuk terus dikembangkan. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan berkoloni pada daerah ruang interseluler dan sistem vascular. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari bagian akar,batang dan daun tanaman sagu yang berpotensi sebagai agens pemacu pertumbuhan tanaman. Bakteri endofit diisolasi dari bagian akar, batang, dan daun. Berdasarkan hasil isolasi yang telah dilakukan didapati 21 isolat bakteri endofit. Kemudian seleksi dilakukan dengan uji hipersensitif dan uji reaksi gram. Hasil seleksi diperoleh 20 isolat bakteri endofit yang bukan patogen. Setelah itu dilanjutkan dengan uji pemacu pertumbuhan tanaman terhadap isolat bakteri endofit. Hasil pengujian tersebut diperoleh tiga isolat bakteri yang berpotensi sebagai pemacu pertumbuhan tanaman yaitu STA1, STA6, dan STA11. Kata kunci: bakteri endofit, pemacu pertumbuhan tanaman, sagu
Rice (Oryza sativa L) is an economically important carbohydrate-producing plant that ranks second only to wheat. In Indonesia, Malaysia, the Philippines, and several other countries, rice is used as a staple food source. The commodity projection is expected in the coming years to grow to reach 70 percent. Blast disease is known as one of the main obstacles in rice cultivation today. Recommended control alternative to the disease was through the utilization of economically beneficial and environmentally friendly resistant varieties. The study used six varieties tested on different cultivated land and designed using a split plot experiment. The results showed that the resistance of the six varieties of rice to blast disease, with the criteria from susceptible to resistance was: Kabir07 (5%) and IPB8G (3%) classified as susceptible, followed by IPB9G 1.8% (moderate resistance), Inpari32 1.8% (moderate resistant), Fas Memeye 1.8% (moderate resistant), and IPB3S 1.2% (resistant). The study found that irrigated rice fields had a higher severity of blast disease than rain-fed rice fields. Keywords: blast disease, resistant variety, rice fields irrigated, rice rainfed lowland ABSTRAK Padi (Oryza sativa L) adalah tanaman penghasil karbohidrat penting secara ekonomi yang menempati peringkat kedua setelah gandum. Di Indonesia, Malaysia, Filipina, dan beberapa negara lain, padi digunakan sebagai sumber makanan pokok. Proyeksi komoditas tersebut diharapkan pada tahun-tahun mendatang tumbuh mencapai 70 persen. Penyakit blas dikenal sebagai salah satu kendala utama dalam budidaya padi saat ini. Alternatif yang direkomendasikan untuk pengendalian terhadap penyakit ini adalah melalui varietas tahan yang bermanfaat secara ekonomi dan ramah lingkungan. Penelitian bertujuan mengevaluasi ketahanan enam varietas padi pada lahan padi sawah dan sawah tadah hujan terhadap penyakit blas. Penelitian ini menggunakan enam varietas diuji pada lahan budidaya yang berbeda dan dirancang menggunakan percobaan petak terpisah. Hasil penelitian menunjukkan bahwa tingkat ketahanan keenam varietas padi terhadap penyakit blas, dengan kriteria dari rentan sampai tahan adalah: Kabir07 (5%) dan IPB8G (3%) tergolong rentan, diikuti oleh IPB9G 1,8% (moderat tahan), Inpari32 1,8% (moderat tahan), Fas Memeye 1,8% (moderat tahan), dan IPB3S 1,2% (tahan). Ditemukan bahwa lahan sawah irigasi memiliki keparahan penyakit blas lebih tinggi dibandingkan dengan sawah tadah hujan. Kata kunci: penyakit blas, sawah tadah hujan, sawah irigasi, varietas resisten
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