This study assessed daily milk yield (DMY), 100-day (MY100), and 305-day (MY305) milk yield, and lactation length (LL) in purebred Ankole cattle and Ankole crossbreds, and the influence of environmental factors on these traits. Milk yield data were obtained for 865 cows and 1234 lactations and analyzed using a mixed linear model. The overall least squares mean of DMY, MY100, and MY305 across breed groups was 2.7 L (N = 1234, SD = 1.7), 262 L (N = 959, SD = 176), and 759 L (N = 448, SD = 439), respectively, while the average lactation length was 256 days (N = 960, SD = 122). All factors included (breed group, season and year of calving, and parity) were significant for yield traits, except season of calving for MY305. First-parity cows had the lowest milk production, and fourth-parity cows the highest. For all traits, pure Ankole cows had the lowest milk yield. Among the crossbreds, there was no significant difference between Ankole × Friesian, Ankole-Jersey mother × Sahiwal sire, and Ankole-Sahiwal mother × Jersey sire, or between Ankole × Sahiwal and Ankole-Sahiwal mother × Sahiwal sire. It was concluded that Ankole crosses with Friesian or Jersey can be beneficial, even under a management system of limited nutrition as in Rwanda.

Highlights The effect of endometritis disease on the milk yield among zero-grazed dairy cows on smallholder farms in rwanda were quantified. The daily milk yield of endometritis positive cows was 15.3% lower compared to endometritis negative cows. The estimated mean MY discarded was 51.4 ± 2.2 litres/cow with a median of 51.5 litres/cow. The percent of total MY loss was much higher (41.6%) among CLE positive cows that received treatment compared to the untreated cows (14.1%). The mean MY loss (litres/cow/day) was 3.2 ± 0.3 for cows having both CLE and SCLE, 2.9 ± 0.2 for CLE only and 1.4 ± 0.7 for cows that had SCLE only.

A chicken genome has several regions with quantitative trait loci (QTLs). However, replication and confirmation of QTL effects are required particularly in African chicken populations. This study identified single nucleotide polymorphisms (SNPs) and putative genes responsible for body weight (BW) and antibody response (AbR) to Newcastle disease (ND) in Rwanda indigenous chicken (IC) using genome-wide association studies (GWAS). Multiple testing was corrected using chromosomal false detection rates of 5 and 10% for significant and suggestive thresholds, respectively. BioMart data mining and variant effect predictor tools were used to annotate SNPs and candidate genes, respectively. A total of four significant SNPs (rs74098018, rs13792572, rs314702374, and rs14123335) significantly (p ≤ 7.6E−5) associated with BW were identified on chromosomes (CHRs) 8, 11, and 19. In the vicinity of these SNPs, four genes such as pre-B-cell leukaemia homeobox 1 (PBX1), GPATCH1, MPHOSPH6, and MRM1 were identified. Four other significant SNPs (rs314787954, rs13623466, rs13910430, and rs737507850) all located on chromosome 1 were strongly (p ≤ 7.6E−5) associated with chicken antibody response to ND. The closest genes to these four SNPs were cell division cycle 16 (CDC16), zinc finger, BED-type containing 1 (ZBED1), myxovirus (influenza virus) resistance 1 (MX1), and growth factor receptor bound protein 2 (GRB2) related adaptor protein 2 (GRAP2). Besides, other SNPs and genes suggestively (p ≤ 1.5E−5) associated with BW and antibody response to ND were reported. This work offers a useful entry point for the discovery of causative genes accountable for essential QTLs regulating BW and antibody response to ND traits. Results provide auspicious genes and SNP-based markers that can be used in the improvement of growth performance and ND resistance in IC populations based on gene-based and/or marker-assisted breeding selection.

No abstract

No abstract