The lactic acid bacteria are involved with food fermentation and in such cases with food spoilage. Considering the need to reduce the lactic acid bacteria growth in meat products, the aim of this work was to enumerated and investigated the lactic acid bacteria present on sliced vacuum-packed cooked ham stored at 4 °C and 8 °C for 45 days by phenotypic and molecular techniques. The quantification showed that the lactic acid bacteria were present from the first day with mean count of 1.98 log cfu/g for the four batches analyzed. The lactic acid bacteria grew rapidly on the samples, and plate counts around 7.59 log cfu/g and 8.25 log cfu/g were detected after 45 days of storage at 4 °C and 8 °C, respectively; storage temperatures studied showed significant influence on the microorganism in study growth. The predominant lactic acid bacteria associated with the spoilage samples at one day of storage includes Lactobacillus sp., the phenotypic overlap Leuconostoc / Weissella sp. and Enterococcus sp. At 45 days of storage at 4 and 8 °C the mainly specie was Lactobacillus curvatus , following by Lactobacillus sakei and Leuconostoc mesentereoides ; the Enterococcus sp. was not present in the samples.

-Parboiled rice bran oil was obtained with supercritical CO 2 at temperatures and pressures varying from 25 to 60ºC and from 150 to 250 bar, respectively. This study was divided into two different parts: initially, the experiments were carried out with one separation step. In the second part, experiments were performed with two separators in series. The temperatures and the pressures of the first separator were 25 and 40ºC, and 100 and 150 bar. The second separator was maintained at 2ºC and 25bar. This procedure results in the precipitation of rice bran oil with different concentrations of tocols in the first and second separators. The extracts obtained were analyzed by HPLC to verify the presence of tocopherols and tocotrienols. Unlike other vegetable oils, rice bran oil contains a larger amount of tocotrienols, specially γ-tocotrienol, than of tocopherols. Finally the extraction curves were modeled by Sovová's method.

No abstract

High sodium intake has been linked with problems of hypertension and in Brazil, it still has above that recommended by the World Health Organization. Meat products contribute 20–30% of sodium intake in the diet, indicating the necessity of reducing the amount of sodium added. The aim of this study was to reduce the sodium content in Toscana sausages by partial replacement of sodium chloride with PuraQ Arome NA4 substitute, and to evaluate the effect on physico-chemical and microbiological parameters and sensory acceptability. Three formulations – one control (T1), and one with 20% (T2) and another with 30% (T3) sodium reduction compared with the control – were produced. For comparative purposes, the physico-chemical, microbiological and sensory parameters were determined. In the proposed formulations with 20% and 30% reduction, the sodium contents were 857.3 mg 100 g-1 (T2) and 790.3 mg 100 g-1 (T3), compared with 926.7 mg 100 g-1 in T1. The produced samples were in accordance with the physico-chemical (moisture, lipid, protein and calcium) and microbiological (Salmonella, coagulase-positive Staphylococcus, and sulphite-reducing Clostridium and Coliforms at 45°C) parameters stipulated by Brazilian law. The analysis of lightness and hue indicated that the sample T3 had a more intense red colour, while T1 had a less intense red colour. In the shear force evaluation, no difference was observed between the samples. The sample T2 was the most preferred in the ordering of preference test; however, in the hedonic scale, there was no difference between the means of sensory values for roast colour, aroma, texture, flavour and overall impression, indicating that all samples had adequate sensory acceptance. Acceptability levels were greater than 74.6% for all attributes evaluated in T1, T2 and T3.

ResumoO objetivo deste trabalho foi avaliar microbiologicamente, físico-quimicamente e sensorialmente uma conserva de tilápia enlatada em óleo vegetal comestível, aproveitando matéria-prima fora do padrão, a fim de oferecer um produto com alto valor agregado. As conservas de tilápia foram levadas a pré-cozimento em vapor fluente por aproximadamente 50 minutos, em seguida adicionado o óleo de cobertura, recravadas e submetidas a dois tratamentos térmicos respectivamente de 15 e 30 minutos em autoclave a 121°C. Após isso foram realizadas as análises microbiológicas, físico-químicas e sensoriais. A avaliação microbiológica mostrou que os produtos apresentavam qualidade satisfatória conforme a legislação. As conservas de tilápia apresentaram seguinte composição proximal: umidade 48,4%; cinzas 3,1%; proteína 12,2%; lipídios 32,7%; cloreto 0,8%. A análise sensorial mostrou que, não houve diferença significativa ao nível de 5% nos diferentes tratamentos. Foi realizado também o teste de esterilidade comercial, onde, os dois tratamentos térmicos foram adequados para manter a qualidade da conserva de tilápia enlatada. Concluiu-se que, as conservas de tilápia, apresentaram qualidade compatível com produtos similares, confirmando a viabilidade de produção deste produto. Palavras-chave: Avaliação, enlatado, pescado, tilápia, tratamento térmico AbstractThe fish is quite sensitive to the deterioration due to the high activity of water and, especially to a near pH of the neutrality. The application of the heat needs a rigorous control of time and temperature. The objective of this work developed meat of tilapia canned in vegetable edible oil, for the use from the nonstandard raw material, in order to offer a product with high collected value. The meat of tilapia were taken to the daily pay-cooking in fluent steam for approximately 50 minutes, when the oil of covering was next added, when re-drove and been subdued to two thermal treatments respectively of 15 and 30 minutes in autoclave 121°C. After that the microbiological analyses were carried out, chemicalphysically and sensory. The microbiological evaluation showed that the products were presenting satisfactory conformable quality to legislation. The pickles of tilapia presented next composition centesimal: moisture 48.4 %; ashes 3.1 %; protein 12.2 %; lipids 32.7 %; chloride 0.8 %. The sensory analysis showed what, did not exist significant difference at the level of 5 % in the different treatments. The test of commercial sterility was carried out also, where two thermal treatments were adapted to maintain the quality of the pickle of tilapia canned. It was ended that, the pickles of tilapia, they presented quality compatible with similar products, confirming the viability of production of this product.