Summary Horse domestication revolutionized warfare and accelerated travel, trade, and the geographic expansion of languages. Here, we present the largest DNA time series for a non-human organism to date, including genome-scale data from 149 ancient animals and 129 ancient genomes (≥1-fold coverage), 87 of which are new. This extensive dataset allows us to assess the modern legacy of past equestrian civilizations. We find that two extinct horse lineages existed during early domestication, one at the far western (Iberia) and the other at the far eastern range (Siberia) of Eurasia. None of these contributed significantly to modern diversity. We show that the influence of Persian-related horse lineages increased following the Islamic conquests in Europe and Asia. Multiple alleles associated with elite-racing, including at the MSTN “speed gene,” only rose in popularity within the last millennium. Finally, the development of modern breeding impacted genetic diversity more dramatically than the previous millennia of human management.

Agriculture first reached the Iberian Peninsula around 5700 BCE. However, little is known about the genetic structure and changes of prehistoric populations in different geographic areas of Iberia. In our study, we focus on the maternal genetic makeup of the Neolithic (~ 5500–3000 BCE), Chalcolithic (~ 3000–2200 BCE) and Early Bronze Age (~ 2200–1500 BCE). We report ancient mitochondrial DNA results of 213 individuals (151 HVS-I sequences) from the northeast, central, southeast and southwest regions and thus on the largest archaeogenetic dataset from the Peninsula to date. Similar to other parts of Europe, we observe a discontinuity between hunter-gatherers and the first farmers of the Neolithic. During the subsequent periods, we detect regional continuity of Early Neolithic lineages across Iberia, however the genetic contribution of hunter-gatherers is generally higher than in other parts of Europe and varies regionally. In contrast to ancient DNA findings from Central Europe, we do not observe a major turnover in the mtDNA record of the Iberian Late Chalcolithic and Early Bronze Age, suggesting that the population history of the Iberian Peninsula is distinct in character.

Agriculture first reached the Iberian Peninsula around 5700 BCE. However, little is known about the genetic structure and changes of prehistoric populations in different geographic areas of Iberia. In our study, we focused on the maternal genetic makeup of the Neolithic (∼ 5500-3000 BCE), Chalcolithic (∼ 3000-2200 BCE) and Early Bronze Age (∼ 2200-1500 BCE). We report ancient mitochondrial DNA results of 213 individuals (151 HVS-I sequences) from the northeast, central, southeast and southwest regions and thus on the largest archaeogenetic dataset from the Peninsula to date. Similar to other parts of Europe, we observe a discontinuity between hunter-gatherers and the first farmers of the Neolithic. During the subsequent periods, we detect regional continuity of Early Neolithic lineages across Iberia, however the genetic contribution of hunter-gatherers is generally higher than in other parts of Europe and varies regionally. In contrast to ancient DNA findings from Central Europe, we do not observe a major turnover in the mtDNA record of the Iberian Late Chalcolithic and Early Bronze Age, suggesting that the population history of the Iberian Peninsula is distinct in character.

Resumen: Se presentan cinco estudios especializados sobre la tumba ostentosa de una joven argárica. Partiendo de esta sepultura singular se tratan aspectos más generales como son: el ritual funerario argárico y el status social de los difuntos; las dataciones radiocarbónicas calibradas de Fuente Álamo, en particular de la ladera sur; los restos óseos humanos hallados en las sepulturas de Fuente Álamo en 1996-1999; las ofrendas animales y de líquidos depositadas en las tumbas; los tejidos prehistóricos. Todas estas aportaciones nos brindan informaciones valiosas sobre la mujer juvenil de la tumba 111 no solamente a la hora de su muerte, sino también sobre el entorno social de su vida. Palabras claves: Fuente Álamo. Edad del Bronce. Dataciones radiocarbónicas. Ritual funerario. Ofrendas orgánicas. Sacrificio animal. Tejido. Rango social. Abstract:Five specialized studies about a young Argaric woman's ostentatious tomb are presented. Starting from this singular burial, several aspects are discussed: the Argaric funerary ritual and the social rank of the dead individuals; the calibrated C-14 dates from Fuente Álamo, particularly from the south slope of the site; the human bones found in the graves at Fuente Álamo in 1996-1999; the offering of animals and liquids in Argaric burials; the prehistoric weaves. All of these contributions bring us valuable information about the young woman buried in grave 111, not only when she died but also how the society where she lived was.

The focus of this study is the analysis of a trepanation performed on the skull of an adult male from the famous Bell Beaker necropolis of Ciempozuelos (Madrid) excavated at the end of the 19 th century. The unusual characteristics of the pottery and other associated grave goods gave rise to the use of the name 'Ciempozuelos' to define the regional Bell Beaker style; this refers to similar finds from other sites on the Meseta of central Spain. Although trepanations from Chalcolithic contexts in the Iberian peninsula are not infrequent, they are unusual in Bell Beaker inhumations. Furthermore, this skull is exceptional not only for the type of trepanation performed, but also for the subsequent cranial deformation apparently resulting from the operation, as well as evidence of a second subsequent trepanation which indicates a very short post-surgical survival period for the individual.