Objective: The aim of the present study is to isolate phenolic compounds from Curcuma zedoaria rhizomes grown in Bogor, West Java, Indonesia, which will enrich phytochemical information from this plant. Methods: C. zedoaria rhizomes were macerated in methanol then followed by increasing polarity partitions with n-hexane, ethyl acetate (EtOAc), and methanol, respectively. EtOAc fraction was further fractionated using various chromatography techniques to yield two isolated fractions, Z1 and Z2. These two isolated fractions were then characterized to determine their compound structures. Results: According to Fourier transform-infrared, ultraviolet-vis, and liquid chromatography–mass spectrometry (MS)/MS data, Z1 fraction was elucidated as curcuminoid derivative, that is, dimethoxycurcumin (DiMC, 1), while Z2 fraction was yielded as a mixture consisted of flavonoid and coumarin derivatives, that is, 3,5,7-trihydroxy-4’-methoxyflavon (kaempferide, 2) and 7-methoxy coumarin (herniarin, 3). Conclusion: This study reveals useful information regarding phenolic constituents of Indonesian C. zedoaria rhizomes. Further research needs to be carried out to purify other compounds contained and to conduct bioactivity assays.

White turmeric (Curcuma zedoaria) is one of plant species belongs to Zingiberaceae from Himalaya, India. Previous research showed that the rhizomes of this plant contained several secondary metabolites such as alkaloid, phenolic, and terpenoid which commonly known to have antibacterial activities. This study aims to investigate the phytochemical of white turmeric rhizomes growth in Indonesia and evaluate its antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis as acne-causing bacteria. In this study, secondary metabolites from Indonesian white turmeric rhizomes were successfully extracted in methanol and partitioned in polarity increasement of several organic solvents. Furthermore, crude and all partitioned extracts were subjected to phytochemical investigation. The ability of crude and ethyl acetate extracts to inhibit the growth of P. acnes and S. epidermidis were evaluated using disk diffusion method. According to the antibacterial activity results, all of samples did not exhibit antibacterial activity against both acne-causing bacteria. The results of this study will provide a useful information regarding the phytochemical and antiacne investigation of Indonesian white turmeric rhizomes.