Eight microsatellite loci were identified and characterized in the commercially important giant freshwater prawn, Macrobrachium rosenbergii. The microsatellite loci were detected by the random screening for dinucleotide repeat units within a partial genomic library developed for the species with biotinylated probes (CA)(15) , (AT)(15) and (GA)(15) . All the eight loci were found to be polymorphic. The number of alleles and observed heterozygosities per locus ranged between three to 16 and 0.22 to 0.71, respectively. These microsatellite markers will be useful for the conservation and management of wild and cultured stocks and population genetic studies of freshwater prawn.
The present study attempts to understand the seasonal and spatial variations in the physico-chemical (temperature, pH, salinity, dissolved oxygen, and nutrients) and productivity characteristics of the northern Arabian Sea off the Indian coast. Samples were collected from four different sites off the Veraval coast. The values of the physical and chemical variables were higher during the summer season, whereas nutrient concentrations were high during the winter season due to the maturity of intake nutrients during post-monsoon and winter convective mixing during the northeast monsoon. The dissolved oxygen (DO) concentration was strongly and positively correlated with the net primary productivity (NPP) and chlorophyll a (Chl-a) content to support productivity along the region. Dissimilarity in study variables was observed between the inshore and offshore locations. Principal component analysis revealed a strong relationship between nutrients and productivity variables (Chl-a and NPP). Nutrient levels were high at inshore sites, which can be attributed to the heavy nutrient load from land-based anthropogenic activities and impact due to natural processes like water mixing, sedimentation, and wave activities. Nutrients were strongly and positively correlated with the productivity variables, i.e., Chl-a and NPP. Chl-a positively correlated with NPP (r = 0.90), which indicates that it is a principle productivity pigment in the marine ecosystem.
Diversification of shrimp farming with marine finfish in a farmer participatory research model was attempted. The study is intended to find an economically viable finfish culture during the fallow period of shrimp farms. The Silver pompano,
Trachinotus blochi i
intercropped with Pacific white shrimp,
Litopenaeus vannamei
culture in coastal shrimp ponds was assessed for growth, survival, and economic viability. During the grow-out period of 100 days, fishes grew from 40.23 ± 1.40 g to 256.56 ± 1.08 g in weight and 12.83 ± 0.19 cm to 25.11 ± 0.09 cm in length. The daily weight gain (DWG) and daily length gain (DLG) were 2.16 g/day and 0.12 cm/day, respectively. Relative growth rate (RGR) and specific growth rate (SGR) recorded for weight was 537.80% in 100 days and 1.85% per day, respectively. Pompano has exhibited its sturdiness and adaptability to the land-based culture system as evidenced by an overall survival percentage of 89.8% including nursery and grow-out phases. The realized feed conversion ratio was 1.94. The culture period of 100 days is found to be enough to attain a desirable harvest size of 250 g. The projected production potential of the experimental demonstration farm of 4500 m
2
water spread area for culture was 16.2 tonnes/cycle with a benefit-cost ratio (BCR) of 1.34 over operational cost. The present participatory trial empirically proved the viability of Silver pompano as an intercrop in coastal shrimp ponds. Thus, the introduction of Pompano in shrimp ponds is recommended and can be promoted for sustainable intercropping with shrimp farming along the Indian coast for improving people’s livelihoods.
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