The recent development of combustion-type nitrogen analyzem capable of h~ relatively huge samples with seml-automatic operation offers a potential replacement for the Kjeldahl method for direct determination of nitrogen. Nitrogen analyses for canola seed, flmmeed, sunflower seed, mustard seed and sofoeans on a LECO (St. Jeseph, MI) FPA28 Nitrogea Analyzer were evaluated against results from the Grain Research Laboratory's (GRL) Kjeldald system. The nitrogen analyzer gave significantly high~ values than the Kjeldald method, resulting in a correction of low values in the GRL Kjeidahl, caused by the inability to use ~ ss catalyst. The standard for result8 from the ,.n=lyzes" WSS comparable to that for the Kjeldahl method. The nitrogen analyzer also was faster than the Kjeldahl method and had less environmental impact. The combustion method h~ replaced the Kjeldald method for routine nitrogen determln~tions in oilseed surveys conducted by the GRL.

. 1994. Effect of wild mustard (Brassica laber) competitioT 9l VigF g9 quality of triazine-tolerant and triazine-susdeptible canola (Brsssl'co nnpus anll Bmssica rapa). Can. J. Plant Research was conducted at two sites near Brandon, Manitoba, in 1990 and l99l to determine the influence of time of removal of wild mustard from triazine-tolerant (TT) or triazine-susceptible (TS)

Yellow‐coated seeds from theBrassica campestris cultivars Tobin and Candle were heavier and contained more oil and protein than the dark‐coated seeds from the same sample. In addition, the yellow‐coated seeds had lower levels of erucic acid, glucosinolates, chlorophyll and crude fiber. These differences were detected in both pedigreed and commercial (producer) samples, but to a larger extent in commercial samples. Reasons for the greater quality differences between yellow‐ and dark‐coated seed could be admixtures of cultivars other than the declared ones of Tobin or Candle or changes in the seed itself as it went from the breeder's stage to the producer stage.

No abstract

Intact glucosinolates in seeds and meals of rapeseed and eanola were isolated and purified on small DEAE ion-exchange columns. After being eluted with potassium sulfate the glucosinelates were hydrolyzed with sulfuric acid to produce thioglucose which was determined as a complex with thymol. The method was compared to a gas liquid chromatography {GLC) procedure which determines aliphatlc glucosinolates. The extra amount of glucosinolates found {ca. 14 ~mol/g oil-free) was equal to the sum of those not determined by GLC. The thymol method had a standard error of ----.3 ~mui/g compared with a standard error of + 1 ~nol/g for the GLC procedure for the same set of 18 samples ranging from 10 ~mol/g to 100/~rnol/g {oil-free, 8.5% moisture basis) of aliphatie glucosino|ates.Many methods have been devised to determine the level of glucosinolates in seeds and other parts of cruciferous pla~ts, and this methodology has been reviewed recently {1, 2). Gas liquid chromatography {GLC) was chosen as the official method for determination of glucosinolate content of rapeseed/canola in Canada 13, 41 and the EEC (5}. Although GLC [or high performance