Objective: Three type of seaweed was chosen for the study namely Gracilaria corticata, Ulva lactuca and Stocheospermum marginatum and was extracted with ethanol. Methods:The fishes [Common carp (Cyprinus carpio) (weight 10±5g)] were divided into five groups. They were infected with A. hydrophila (1.6 × 10 4 CFU/fish). The infected three groups were injected with seaweed extract (0.20 mg) suspended in saline solution. One group was injected only saline control the other one group was injected with ciprofloxacin (0.20 mg) (standard) suspended in saline. The experiment was carried out for 28 d. Every seven days interval the fishes were injected with seaweed extract and blood parameters of RBC, WBC, HB, Ht, MCV, MCH, and MCHC were recorded. Results:The MCHC value of Gracilaria corticata was recorded in 28 th day (29.28±1.15) g/l. In Stocheospermum marginatum, the MCHC value was found in (27.19±1.62) g/l on 28 th day. The Ulva lactuca had a MCHC value of (26.80±2) g/l on 28 th day. Conclusion:From this study, we can understand that the fish injected with seaweed extracts as good Immunostimulants properties.

No abstract

Heteropneustes fossilis is considered as a cheap and manageable fish species for aquaculture. Aeromonas hydrophila was found as one of principal pathogenic bacteria causing diseases among the stinging catfishes. In the present study, herbal extracts like Coleus aromaticus (CA), Mentha piperita (MP), Leucas aspera (LA) and their combo (Tri Herbal Extract -THE) were utilized in enhancing the immunity of catfishes by intraperitoneal injection along with FKC vaccines against A. hydrophila. FKC vaccines with THE and CA were found to trigger the non-specific immunity, which was analyzed using hematological, biochemical and immunological studies.