ABSTRACT:A simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Metformin and Glipizide. The method involved estimation of Metformin and Glipizide by simultaneous equation at 272nm and 232nm respectively in their solution in water. This method was validated with respect to linearity, accuracy, precision, LOD and LOQ. Beer's law obeyed in the concentration range of 5-25µg/ml and 20-50µg/ml for Metformin and Glipizide respectively with the correlation coefficient of above 0.99. Limit of detection and quantification values were determined to be 0.214μg/ml and 0.649μg/ml for Metformin and 0.608μg/ml and 1.854μg/ml for Glipizide respectively. Mean recovery of Metformin and Glipizide were found to be in the range of 98-102% signifies the accuracy of the method. The method was found to be precise as %RSD was less than 2.

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A new simple, rapid, specific, accurate, precise and novel Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of Sitagliptin Phosphate in the pharmaceutical dosage form. The chromatographic separation for Sitagliptin was achieved with mobile phase containing methanol, Thermoscientific C18 column, (250x4.6 particle size of 5µ) at room temperature and UV detection at 248 nm. The compounds were eluted in the isocratic mode at a flow rate of 1ml/min. The retention time of Sitagliptin was 1.91min. The above method was validated in terms of linearity, accuracy, precision, LOD and LOQ in accordance with ICH guidelines.

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A simple, accurate stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Rosuvastatin and Ezetimibe has been developed and validated. Chromatographic analysis was performed on Shimadzu LC-20AD Waters X-bridge C18, 5µm (4.6 x 250mm) column at ambient temperature and a UV detector, using Acetonitrile : Methanol (30:70% v/v) as the mobile phase, with a flow rate of 1ml/min and detection wavelength at 230nm. The retention times of rosuvastatin and ezetimibe were 2.0mins and 3.0mins respectively. Calibration graphs were linear over the concentration ranges of 5-25µg/ml for both rosuvastatin and ezetimibe. The accuracy of proposed method was determined by recovery studies and was found to be 98% to 102% for both rosuvastatin and ezetimibe. To establish the stability of the method, degradation studies were carried out in acid, base, oxidation, thermal and photolytic (UV chamber) conditions and the percent degraded was calculated.