Twenty Pasundan cows were used in this study to know the effect of synchronization using prostaglandins and hormone gonadotropins on the picture of erythrocytes, leukocytes, and pasundan cow hemoglobin levels during pregnancy. The Pasudan heifers experimented with estrus using a combination of prostaglandin hormone (PGF2α) as much as 5 ml per head and gonadotropin realising hormone (GnRH) as much as 2.5 ml per head intramuscular to homogenize fertility conditions and improve fertility. The estrus mother cow is immediately carried out artificial insemination as much as 2 times with an interval of 6 hours. All test cows were given forage basalt food and adlibitum drinking water. Pregnancy examination is carried out on the 60th day and the 150th day of post-insemination using the rectal palpation method. Changes observed in the form of hematological concentrations include erythrocytes, leukocytes, and hemoglobin. The results showed that the concentration erythrocytes, leucocyte, and hemoglobin for Garut region respectively was 6.24±0.61 (million/μl); 11.54±0.25 (thousand/(μl); 11.54±0.61 (g/dl) higher than Bogor in a row was 5.99 ± 0.64 (million/μl); 11.46± 1.41 (thousand/(μl); 11.13 ± 0.60 (g/dl)). The results of the variance analysis showed that the synchronization of estrus with prostaglandins (PGF2α) and the gonadotropin releasing hormones (GnRH) did not differ markedly (P>0.05), between the concentrations of erythrocytes, leucocytes, and hemoglobin during the gestation period with cows that were not pregnant. It concluded that the synchronization treatment of estrus with prostaglandins (PGF2α) and gonadotropin releasing hormones (GnRH) had no effect on the profile of hematological concentrations during the gestational period (60 days and 150 days) and was no different from Pasundan heifers that are not pregnant.

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Abstract. This study was conducted to determine the reproductive performance of various breeds of Sentul chicken. The present research was assigned in an experiment model with a completely randomized design (CRD), with 5 breeds of Sentul chicken as treatments, namely Abu Sentul chicken = SA; Batu Sentul Chicken=SB; Emas Sentul Chicken=SE; Debu Sentul Chicken=SD; and Geni Sentul Chicken=SG. Each treatment was repeated 5 times with 4 female and 1 male birds each, thus the total number of chicken were 100 females and 25 males with an average initial female weight was 1,336.05±84.99 g and male weight was 2,050.53±121.78 g. The experiment was conducted for 14 weeks started from 20 week old until 34 week old. Variables measured were spermatozoa production, fertility, and hatchability. Data were subjected to analysis of variance and HSD test. Results showed that various breeds of Sentul chicken had no significant effect (P>0.05) on spermatozoa production, and had significant effect (P<0.05) on fertility and hatchability. It can be concluded that spermatozoa production of various breeds of Sentul chicken are relatively similar, while fertility and hatchability of Emas chicken was lower than that of Abu Sentul chicken and Batu Sentul Chicken. Key words:Reproductive performance, spermatozoa production, fertility, hatchability, Sentul chicken Abstrak. Penelitian ini dilakukan untuk mengkaji kinerja reproduksi berbagai jenis ayam Sentul. Penelitian ini menggunakan metode eksperimen dengan rancangan acak lengkap (RAL), Perlakuan adalah 5 jenis ayam Sentul yang terdiri atas ayam Sentul Abu = SA; ayam Sentul Batu = SB; ayam Sentul Emas = SE; ayam Sentul Debu = SD; dan ayam Sentul Geni = SG. Setiap perlakuan diulang 5 kali masing-masing terdiri dengan 4 betina dan 1 ekor jantan, sehingga jumlah total ayam sentul ada 100 ekor betina dan 25 ekor jantan dengan berat awal rata-rata ayam betina adalah 1.336,05±84,99 g dan berat ayam jantansebesar 2.050,53±121,78 g. Penelitian dilakukan selama 14 minggu mulai dari umur 20 minggu sampai 34 minggu. Variabel yang diukur adalah produksi spermatozoa, fertilitas, dan daya tetas. Data yang diperoleh kemudiandianalisis variasi dan dilanjutkan uji BNJ. Hasil penelitian menunjukkan bahwa berbagai jenis ayam Sentul tidak berpengaruh (P>0,05) terhadap produksi spermatozoa, dan berpengaruh nyata (P<0,05) pada fertilitas dan daya tetas. Penelitian ini dapat disimpulkan bahwa produksi spermatozoa berbagai jenis ayam Sentul relatif sama, sedangkan fertilitas dan daya tetas ayam Sentul Emas lebih rendah dibandingkan ayam Sentul Abu dan ayam Sentul Batu.

Penelitian bertujuan untuk mengetahui pengaruh interaksi antara rasio pengencer ringer laktat-putih telur dan penyimpanan selama 48 jam pada suhu 5 C terhadap motilitas dan abnormalitas spermatozoa ayam kampung. Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan pola faktorial 6 x 5 dengan faktor R yaitu rasio pengencer ringer laktat dengan putih telur (r0=kontrol, r1=ringer laktat, r2=ringer laktat 75% + putih telur 25%, r3=ringer laktat 50% + putih telur 50%, r4=ringer laktat 25% + putih telur 75%, dan r5=putih telur) dan faktor T yaitu penyimpanan selama 48 jam pada suhu 5 C (t1=1 jam, t2=12 jam, t3=24 jam, t4=36 jam, t5=48 jam) masing-masing perlakuan diulang empat kali. Hasil penelitian menunjukkan bahwa masing-masing faktor memberikan pengaruh terhadap motilitas dan abnormalitas spermatozoa ayam kampung (p<0,05). Hasil menunjukkan motilitas terbaik spermatozoa ayam kampung dihasilkan oleh perlakuan ringer laktat dengan penyimpanan selama 12 jam (82,5%). Selanjutnya, abnormalitas terbaik spermatozoa ayam kampung (8,5%) dihasilkan oleh perlakuan ringer laktat dengan penyimpanan selama satu jam. Persentase abnormalitas spermatozoa dari semua perlakuan menunjukkan kurang dari 20%. Dari hasil penelitian ini maka dapat disimpulkan bahwa spermatozoa ayam kampung yang diencerkan dengan ringer laktat dengan penyimpanan pada suhu 5 C dapat digunakan untuk inseminasi sampai 12 jam.Kata kunci: spermatozoa, ayam kampung, pengencer, ringer laktat, putih telur (Motility and Abnormality of Kampong Rooster Sperm Diluted with Different Kinds of Extenders and Stored at 5 C for 48 Hours)The purpose of this research was to determine the interaction effects between types of extender and storage time on sperm motility and abnormality of kampong rooster. This experiment was implemented following a 6x5 factorial design, in which the ratio between Ringer Lactat and egg albumen i.e. r0= control; r1=ringer lactat; r2=75% ringer lactate+25% egg albumen; r3=50% ringer lactate+50% egg albumen; r4=25% ringer lactate+75% egg albumen, and r5=egg albumen as the first factor and storage time which consisted of t1=1 hour; t2=12 hrs; t3=24 hrs; t4=36 hrs as the second factor. Each of the treatment was replicated four times. Results indicated that the types of extender and storage time had no significant interaction (p>0.05) on the percentage of motility and abnormality of kampong rooster sperm. However, each of the treatment had a significant effect (p<0.05) on the motility and the abnormality of kampong rooster sperm. The highest value of sperm motility (82.5 %) was obtained from a ringer lactat extender. This sperm motility value has a significant difference (p<0.05) from the 5 other types of extender. The lowest value of sperm abnormality (8.5%) was obtained from an hour of storage time. This value has a significant difference (p<0.05) than the 4 other treatments. However the percentage of abnormal sperm from all the treatments is less than 20 percent. It could be concluded that kampong rooster sperm which diluted with ringer lactate stored at 5 0C can still be utilized for Insemination not until 12 hours of stored.Keywords: sperm, kampong rooster, extender, ringer lactatee, egg albumen

Twenty cows head were used in research to know aspect reproduce and economic estimation in cow which innovated by reproduction technology. All of cows in allocated into two groups at random each 10 heads. The first group come from farmer of co-operation member of Ciamis was used as control without innovated by reproduction technology, that mean the cow was naturally of oestrous and inseminated with frozen semen not a result of separation sex. The second group come from farmer group "lumba-lumba" innovated with oestrous synchronization which was injected prosolvin of 7.5 mg per headl by intramuscular (i.m.) and super ovulated with injected fertagyl of 150 mg per tail by i.m., and then it oestrous was inseminated with frozen semen result of sex separation. Research result indicates that technically there were difference of performance reproduce in cow which is innovated by reproduction technology than control. It concluded that the improvement of reproduction efficiency in cow after innovated by technology of reproduction in term of type of birth in twin and increasing of male sex up to 12 percent. It is in line with the economic estimation of cow which is technological application of reproduction improves generating revenue up to 22.35 percent per month.