This research aimed to determine the superiority of each free-range rooster based on mass movements, abnormalities, and motility of spermatozoa. This research using 10 free-range roosters. The roosters were 10-12 months old, which were known based on 2-3 cm of spurs size and 2-2.5 kg of weight. This research used some equipments consist of individual coop, feeder, bottle, eppendorf tube, beaker glass, pipette, microscope, bunsen burner, object glass, and hand-tally counter. This research used some materials consist of semen, tissue, 2% eosin, and NaCl for diluter. This research used non parametric design (One-way ANOVA and Kruskall Wallis). This result showed that the cement volume of each rooster was significantly different (P≤ 05), while the pH, color, consistency, smell, abnormality, and motility of each rooster were not significantly different (P≥0.05). The qualitative and quantitative analysis resulted the total breeding value of each rooster, they were rooster 2 (42), 6 (42), 7 (42), 8 (42), 9 (42), and 10 (42). Based on its total breeding value, chosen the best roosters were rooster 2,