"Coalhada" is a type of fermented milk (made with Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris) very popular in Brazil, which is widely consumed due to its pleasant texture and mild taste and aroma. Its acceptance is possible to increase by adding a fruit preparation. Fermented milk is the most common food matrix used for delivery probiotic bacteria, which are able to survive to the passage through the gastrointestinal tract, reaching the colon in high concentration where they can effectively bind and subsequently, improve the body functions as a whole. In this study, two formulations of "Coalhada" were developed which resemble a sundae (in which, the fruit preparation stays on the botton of the package and the fermented milk on the upper part) used for a blueberry preparation. The formulation F1 was the control, with no addition of probiotic bacteria and to the formulation F2 the probiotic Lactobacillus paracasei was added. The formulations were evaluated over 14 days of storage under refrigeration. The characteristics evaluated in the product were: moisture, pH, acidity, lipids and ash content and all of them were not significantly affected (p > 0.05) by the addition of the probiotic bacteria. However, the addition of Lactobacillus paracasei significantly decreased (p < 0.05) the syneresis of the product during the refrigerated storage. Blueberry preparation had a high antioxidant activity which was 330.8 µmol TEAC 100 g -1 and the total phenolic compounds content was 36.65 mg GAE 100 g ) during the 14 days of storage, but the shelf life of the two formulations was around seven days, since in the second week of storage the counts of yeasts and molds reached 10 2 CFU g -1 (which is the maximum allowed by Brazilian legislation). It is important to note that there was no addition of preservatives to the blueberry preparation, although the Brazilian legislation allows this and it could extend the shelf life of the final product, because yeasts and molds found in the product, probably comes from the fruits. Concluding that it was feasible to use blueberries in functional fermented milk such as "sundae-type Coalhada" and that it was a good way to innovate and to add value to the final product.

Despite lower energy intakes with age, elderly have higher requirements for several nutrients, making them vulnerable to deficiencies that further aggravate aging chronic conditions. To manage this problem, new food formulations are needed to address the nutritional needs of the worldwide growing aging population. In the present work, we develop an innovative functional fruit/vegetable beverage for the elderly using coconut water, orange juice and carrot juice. The beverage was enriched with vitamin A and C and supplemented with omega-3 fatty acids and inulin to provide important nutritional elements to the target consumers of this study. Osmolality classified the beverage as hypertonic. Heat (TT), supercritical carbon dioxide (SC-CO 2 ) and dimethyl dicarbonate (DMDC) treatments were applied as preservation methods, and the responses of the functional and enriched components and enzymes were evaluated. Alicyclobacillus acidoterrestris ATCC 49025 and a surrogate Escherichia coli, ATCC 25922, were inoculated in the beverage to induce spoilage and verify the effectiveness of inactivation processes on the cells. The DMDC process reduced E. coli to undetectable levels and exhibited a greater reduction of A. acidoterrestris vegetative cells, molds and yeasts. The conditions applied in the process with SC-CO 2 did not show satisfactory results in reducing juice microbiota. Loss of vitamin C and A was mainly associated with the SC-CO 2 treatment. The beverage received a positive acceptability evaluation among the tasters in the sensory analysis and may be introduced as a new vehicle for the consumption of functional compounds, especially by vegans and/or vegetarians and lactose-intolerant elderly.

ResumoCom as tecnologias de comunicação e a globalização, aumentou a preocupação com uma alimentação mais saudável e neste contexto surgiram os alimentos funcionais. A coalhada é um tipo de leite fermentado com características similares as do iogurte, mudando as bactérias láticas adicionadas (Lactococcus lactis ssp. lactis e Lactococcus lactis ssp. cremoris) e a temperatura de fermentação (37 ºC). Os leites fermentados têm sido cada vez mais consumidos devido ao sabor, odor e textura agradáveis e a aceitação destes produtos aumenta ainda mais quando são acrescidos de polpa ou preparados de frutas. O mirtilo é rico em compostos fenólicos como as antocianinas que agem como antioxidantes, sendo considerada a fruta da longevidade. Os leites fermentados são ótimos veículos para adição e consumo de culturas probióticas, que são microorganismos capazes de sobreviver a passagem pelo estomago, chegando ao cólon do intestino em concentrações elevadas com capacidade de colonizá-lo e melhorar o funcionamento do intestino. Neste trabalho foram desenvolvidas duas formulações de coalhada tipo Sunday com preparado de mirtilo, sendo uma formulação com adição da cultura probiótica Lactobacillus paracasei e a outra sem. Estas duas formulações foram avaliadas durante 14 dias de armazenamento refrigerado. Os resultados mostraram que características tais como: a umidade, pH, acidez, conteúdo