Background: The world wide increasing popularity of the energy drinks and the lack of information about their possible hazardous effects on health is a matter of controversy and research. The aim of this study is to assess the histological and histochemical effects of energy drinks on the pancreas of adult male albino rats and the possible protective effect of omega-3. Material and Methods: Fifty adult male albino rats were randomly divided into 4 groups. First group is control. Second group (Omega-3 treated) rats received omega-3 at a dose of 300 mg /kg/day orally for 4 weeks. Third group (Red Bull treated) rats received Red Bull at a dose of 10 mg/kg/day orally for 4 weeks then they were randomly subdivided into two equal subgroups: IIIA, rats were sacrificed after 24 h of the last dose and in IIIB (Recovery group), rats were sacrificed after 4 weeks of the last dose .Fourth group (Omega-3 and Red Bull treated group) rats received Red Bull at a dose of 10mg/kg/ day and Omega-3 at a dose of 300mg/kg/day for 4 weeks. At sacrifice, blood samples were drawn for biochemical study and pancreas specimens were prepared for histological and histochemical study. Results: Energy drink had no significant effect on the animal weight (P = 0.055), but there was highly significant increase in the pancreatic weight (P = 0.001) and in mean blood glucose level (P = 0.000). There were signs of β cells overstimulation. Histological and histochemical study of the pancreatic sections revealed multiple deleterious effects of the energy drink on the acinar and the islet cells. These changes were reversible as shown in the recovery group. Co-administration of Omega-3 showed marked protection of the pancreatic acini and the islets of Langerhans. Conclusion: Omega-3 administration has a highly protective effect on the pancreatic tissue against the hazardous effects of the energy drinks.

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Introduction: 5-fluorouracil is a potent antimetabolite chemotherapeutic medication used for treatment of various malignancies . Using this agent result in toxic effect mainly in the lining of digestive tract. To ameliorate 5 FU toxic effect ginger was implicated in this study. Aim of the Work:To evaluate the microscopic effect of 5-fluorouracil on gastric fundus mucosa in rat models . and to study the possible beneficial role of ginger in the ameliorations of these changes. Material and Methods: Forty-eight adult male albino rates were divided into four groups: group I (control), group II received ginger orally in a dose of 200 mg/kg/day , group III received 5-fluorouracil (15 mg/kg) intraperitoneally and group IV received 5 FU and Ginger as previous doses for 5 days. The fundic part of stomach was excised and subjected to light and electron microscopic studies. Results: In 5 FU treated group, the fundic gastric mucosa showed multiple areas of tissue loss , congested dilated blood vessels and extravasated red blood cells and mononuclear infiltration were seen between fundic gland cells. Parietal cells showed ballooned appearance, vacuolated cytoplasm, and pykontic nuclei. PAS/ Alcian blue stained sections showed marked reduction in both alcian blue and PAS positive reaction. There was strong positive cytoplasmic reaction in the apical and middle parts of the fundic glands for caspase 3 while large number of parietal cells were stained positive for Cox 2. These histological findings were improved after ginger administration. Histomorphometric study revealed significant weight reduction, increased mucosal ulcer depth, decreased mucosal thickness and increased number of parietal cells in 5 FU treated group when compared to control group. Conclusion: 5FU causes gastric damage in rat model. Ginger supplementation could be used to alleviate the damaging pathological effect on gastric fundus.

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