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The careful study of cell-based lung repair and regeneration ex vivo may one day provide us with the necessary tools to create a patient-derived alternative to cadaveric donor lungs for transplantation. Many parameters must be monitored and optimized to advance this aim. The use of rat lungs as a small-scale model for lung regeneration is an efficient way to develop the key improvements required for optimal tissue repair and regeneration. In this study, we report the use of a novel high-throughput, automated, multichannel lung bioreactor system, which allows for culture and analysis of rodent scale isolated lungs. In this model, five decellularized rat lungs can be repopulated with human primary endothelial and epithelial cells, cultured under varying perfusion and ventilation parameters in parallel, and analyzed for standardized endpoints. As a proof of principle, we report a multiphase organ culture protocol, which achieves consistent tissue regeneration across lungs at multiple points during culture, and further promotes overall tissue maturation through the application of lung ventilation.

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