No abstract

Mangifera casturi Kosterm., a mango plant from Kalimantan Selatan, Indonesia, has limited genetic information, severely limiting the research on its genetic variation and phylogeny. We collected M. casturi’s genomic information using next-generation sequencing, developed microsatellite markers and performed Sanger sequencing for DNA barcoding analysis. These markers were used to confirm parental origin and genetic diversity of M. casturi hybrids. The clean reads of the Kasturi accession were assembled de novo, producing 259 872 scaffolds (N50 = 1 445 bp). Fourteen polymorphic microsatellite markers were developed from 11 040 microsatellite motif-containing sequences. In total, 58 alleles were produced with a mean of 4.14 alleles per locus. Microsatellite marker analysis revealed broad genetic variation in M. casturi. Phylogenetic analysis was performed using internal transcribed spacers (ITS), matK, rbcL, and trnH-psbA. The phylogenetic tree of chloroplast markers placed Kasturi, Cuban, Pelipisan, Pinari, and Hambawang in one group, with M. indica as the female ancestor. Meanwhile, the phylogenetic tree of ITS markers indicated several Mangifera species as ancestors of M. casturi. Thus, M. casturi very likely originated from the cross-hybridization of multiple ancestors. Furthermore, crossing the F1 hybrids of M. indica and M. quadrifida with other Mangifera spp. may have generated much genetic variation. The genetic information for M. casturi will be a resource for breeding improvement, and conservation studies.

Rambai (Baccaurea motleyana) is one of the underutilized fruits native to Indonesia. Rambai has high antioxidant activities containing phenolic, flavonoid, and anthocyanin compounds, secondary metabolite compounds derived from sugar metabolism. The sugar metabolism involved several related genes. This research aimed to characterize genes putatively involved in sugar metabolism in Rambai. Six sugar gene families were identified from 37 077 contigs of the assembled-transcriptome database against to UniProt database using the BLASTX program. The six sugar-related genes were characterized involved nine contigs of sucrose-phosphate synthase (SPS), three contigs of sucrose-phosphatase (SPP), 14 contigs of sucrose synthase (SUS), 19 contigs of alkaline/neutral invertase (INV), one contig of cytosolic invertase (CINV) and five contigs of beta-fructofuranosidase (CWINV). This research aims to give a comprehensive study of the sugar metabolism mechanism in B. motleyana. The data also revealed the genes that encoded the enzymes that were putatively involved in sugar metabolism.

The information of secondary metabolite compound from underutilized Indonesian fruits are still limited including rambai ( Baccaurea motleyana Müll.Arg.), nangkadak ( Artocarpus nangkadak or A. heterophyllus x A. integer ), rambutan ( Nephelium lappaceum L.) and Sidempuan salak ( Salacca sumatrana Becc.). To identify the secondary metabolite, we used GC-MS (gas chromatography-mass spectrometry) and LC-MS (liquid chromatography-mass spectrometry) analyses. The accessions/varieties numbers used in this analysis including two accession for rambai, three accessions for nangkadak, four varieties for rambutan and three accessions for Sidempuan salak. All sample were collected from edible part such arilode/carpel and also rind for only rambutan. Based on, spectral data showed common and specific secondary metabolite compounds in each commodity. Preliminary GCMS analysis from the dataset obtained specific secondary metabolites contained in rambai; Decanoic acid, 1-Decene, Methyl salicylate and Stearyl alcohol, nangkadak; β-Cyclocitral, 2-Furanmethanol and Linoleic acid, rambutan; Citraconic anhydride, 3,5-Dideuteropyridine-4-carboxylic acid, Isobutyl formate and n-Methyl-D3-Aziridine, and Sidempuan salak; 5-Formyl-2-furfurylmethanoate, 2-Methoxy-4-vinylphenol and Tiglic acid.