The taxonomic positions of two novel actinomycetes, designated strains BNT558T and SM3501T, were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Streptomyces . The whole-cell hydrolysates of the two strains contained ll-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H6) and MK-9(H8) for strain BNT558T and MK-9(H8) and MK-9(H6) for strain SM3501T. Major fatty acids of the strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipid profile of strain BNT558T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, one unidentified glycolipid and one unidentified aminophospholipid, while that of strain SM3501T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, three unidentified atypical aminolipids, one unidentified aminolipid and two unidentified glycolipids. The G+C contents of the genomic DNA were 70.2 and 69.6 mol% for strains BNT558T and SM3501T, respectively. 16S rRNA gene sequence data supported the classification of the isolates in the genus Streptomyces and showed that they formed two distinct branches within the genus. Based on almost-complete 16S rRNA gene sequences, strain BNT558T was related most closely to Streptomyces albiaxialis NRRL B-24327T and strain SM3501T was related most closely to Streptomyces cacaoi subsp. cacaoi NBRC 12748T. DNA–DNA relatedness between each of the isolates and its closest phylogenetic neighbours showed that they belonged to distinct species. The two isolates were readily distinguished from one another and from the type strains of the other species classified in the genus Streptomyces based on a combination of phenotypic and genotypic properties. Based on the genotypic and phenotypic evidence, strains BNT558T and SM3501T belong to two novel species in the genus Streptomyces , for which the names Streptomyces iconiensis sp. nov. (type strain BNT558T = KCTC 29198T = DSM 42109T) and Streptomyces smyrnaeus sp. nov. (type strain SM3501T = KCTC 29214T = DSM 42105T) are proposed, respectively.
A novel halotolerant actinomycete, designated strain BNT52T , was isolated from soil collected from Cihanbeyli Salt Mine in the central Anatolia region of Turkey, and examined using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the genus Amycolatopsis and formed a distinct phyletic line in the 16S rRNA gene tree. Strain BNT52 T was most closely related to Amycolatopsis nigrescens CSC17Ta-90 T (96.7 %), strains of species of the genus Amycolatopsis were lower than 96.2 %. The isolate grew at 20-37 6C, pH 6-12 and in the presence of 0-10 % (w/v) NaCl. The cell wall of the novel strain contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids were iso-C 16 : 0 2-OH and iso-C 16 : 0 . The predominant menaquinone was MK-9(H 4 ). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylmethylethanolamine. The genomic DNA G+C content was 68.8 mol%. On the basis of the data from this polyphasic taxonomic study, strain BNT52 T represents a novel species within the genus Amycolatopsis for which the name Amycolatopsis cihanbeyliensis sp. nov. is proposed (type strain BNT52 IntroductionThe genus Amycolatopsis, first established by Lechevalier et al. (1986), belongs to the family Pseudonocardiaceae and is a member of the suborder Pseudonocardineae (Embley et al., 1988;Warwick et al., 1994;Stackebrandt et al., 1997). The members of the genus are Gram-reaction-positive, nonacid-fast, non-motile actinomycetes that form branched vegetative hyphae that undergo fragmentation into rod-like and squarish elements. The genus contains halophilic, alkaliphilic, mesophilic, thermophilic and pathogenic species (SaintpierreBonaccio et al., 2005;Lee, 2006;Lee et al., 2006;Groth et al., 2007). The species of the genus Amycolatopsis have mesodiaminopimelic acid, arabinose and galactose in the wall peptidoglycan (wall chemotype IV sensu Lechevalier & Lechevalier, 1970), fatty acids rich in iso-and anteiso-branched components and a lack of mycolic acids (Takahashi, 2001). The predominant menaquinone type is MK-9(H4); phosphatidylethanolamine is a diagnostic phospholipid (type II sensu Lechevalier et al., 1977) and the G+C content of genomic DNA ranges from 66 to 73 mol%. The aim of the present polyphasic study was to clarify the taxonomic position of the novel strain BNT52 T of the genus Amycolatopsis, isolated from a soil sample, collected from Cihanbeyli Salt Mine, in Turkey.During research on biodiversity of culturable actinomycetes from a soil sample collected from Cihanbeyli Salt Mine (GPS coordinates for the sampling site are 38 u 459 500 N 33 u 099 260 E), located in the central Anatolia region, actinomycete strain BNT52T was isolated on modified Bennett's agar (Jones, 1949) supplemented with 5 % NaCl (w/v), which had been inoculated with a soil suspension and incubated at 28 u C for 21 days. The organism was maintained on modified Bennett's agar slopes containing ...
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