Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine

Abstract

A novel halotolerant actinomycete, designated strain BNT52T , was isolated from soil collected from Cihanbeyli Salt Mine in the central Anatolia region of Turkey, and examined using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the genus Amycolatopsis and formed a distinct phyletic line in the 16S rRNA gene tree. Strain BNT52 T was most closely related to Amycolatopsis nigrescens CSC17Ta-90 T (96.7 %), strains of species of the genus Amycolatopsis were lower than 96.2 %. The isolate grew at 20-37 6C, pH 6-12 and in the presence of 0-10 % (w/v) NaCl. The cell wall of the novel strain contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids were iso-C 16 : 0 2-OH and iso-C 16 : 0 . The predominant menaquinone was MK-9(H 4 ). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylmethylethanolamine. The genomic DNA G+C content was 68.8 mol%. On the basis of the data from this polyphasic taxonomic study, strain BNT52 T represents a novel species within the genus Amycolatopsis for which the name Amycolatopsis cihanbeyliensis sp. nov. is proposed (type strain BNT52 IntroductionThe genus Amycolatopsis, first established by Lechevalier et al. (1986), belongs to the family Pseudonocardiaceae and is a member of the suborder Pseudonocardineae (Embley et al., 1988;Warwick et al., 1994;Stackebrandt et al., 1997). The members of the genus are Gram-reaction-positive, nonacid-fast, non-motile actinomycetes that form branched vegetative hyphae that undergo fragmentation into rod-like and squarish elements. The genus contains halophilic, alkaliphilic, mesophilic, thermophilic and pathogenic species (SaintpierreBonaccio et al., 2005;Lee, 2006;Lee et al., 2006;Groth et al., 2007). The species of the genus Amycolatopsis have mesodiaminopimelic acid, arabinose and galactose in the wall peptidoglycan (wall chemotype IV sensu Lechevalier & Lechevalier, 1970), fatty acids rich in iso-and anteiso-branched components and a lack of mycolic acids (Takahashi, 2001). The predominant menaquinone type is MK-9(H4); phosphatidylethanolamine is a diagnostic phospholipid (type II sensu Lechevalier et al., 1977) and the G+C content of genomic DNA ranges from 66 to 73 mol%. The aim of the present polyphasic study was to clarify the taxonomic position of the novel strain BNT52 T of the genus Amycolatopsis, isolated from a soil sample, collected from Cihanbeyli Salt Mine, in Turkey.During research on biodiversity of culturable actinomycetes from a soil sample collected from Cihanbeyli Salt Mine (GPS coordinates for the sampling site are 38 u 459 500 N 33 u 099 260 E), located in the central Anatolia region, actinomycete strain BNT52T was isolated on modified Bennett's agar (Jones, 1949) supplemented with 5 % NaCl (w/v), which had been inoculated with a soil suspension and incubated at 28 u C for 21 days. The organism was maintained on modified Bennett's agar slopes containing ...

Amycolatopsis anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior

“…1) method revealed that strain EGI 650086 T was placed in a distinct subclade from the closest related strain A. nigrescens JCM 14717 T . These phylogenetic tree patterns are similar to several previous reports as described by Tatar et al [6], Duangmal et al [12], Zucchi et al [52], Zhang et al [53], Camas et al [54] and Jamjan et al [55]. The phylogenomic tree based on the concatenated alignment of 22 marker genes, which provided further evidence showed that strain EGI 650086 T belonged to the genus Amycolatopsis, and EGI 650086 T formed a distinct branch within the cluster encompassing the members of the genus Amycolatopsis (Fig.…”

“…The genus was first proposed by Lechevalier et al [1] and the genus description was emended by Lee [2] in 2009, and then by Tang et al [3] in 2010, further recently by Nouioui et al [4] in 2018. Members of the genus Amycolatopsis have been isolated from a wide variety of environmental sources, such as soil [5], salt mine [6], salt lake [3], cave [7], ocean sediment [8], catacomb [9], human sources [10] and plant tissues [11]. At the time of writing this article, the genus Amycolatopsis encompasses 76 species with validly published names and four subspecies (LPSN, http://www.…”

Streptomyces seymenliensis sp. nov., isolated from soil

“…Saline lakes such as Tuz (Salt) Lake, which is the second largest lake of Turkey, have been a valuable source of novel microorganisms (Tatar et al 2013). Formation of Tuz (Salt) Lakebegan with tensional movements during the Late Cretaceous, followed by compressional episodes that, during the Late Eocene, resulted in the isolation of the basin from the open sea (Dirik and Erol 2003).…”

Production, purification, characterization and usage of a detergent additive of endoglucanase from isolated halotolerant Amycolatopsis cihanbeyliensis mutated strain Mut43

“…In the present study, the halotolerant Amycolatopsis cihanbeyliensis, which was isolated from the Cihanbeyli Salt Mine in Turkey (Tatar et al 2013), was mutated with n-methyl-n-nitro-n-nitrosoguanidine (NTG) and UV to improve endoglucanase production. The effect of medium components and process conditions on endoglucanase production by mutated strain in batch culture was determined.…”