Hepatic triglyceride (HTG) accumulation from peripheral dietary sources and from endogenous de novo lipogenesis (DNL) was quantified in adult Sprague-Dawley rats by combining in vivo localized 1 H MRS measurement of total hepatic lipid with a novel ex vivo 2 H NMR analysis of HTG 2 H enrichment from 2 H-enriched body water. The methodology for DNL determination needs further validation against standard methodologies. To examine the effect of a high-fat diet on HTG concentrations and sources, animals (n ¼ 5) were given high-fat chow for 35 days. HTG accumulation, measured by in vivo 1 H MRS, increased significantly after 1 week (3.85 W 0.60% vs 2.13 W 0.34% for animals fed on a standard chow diet, P < 0.05) and was maintained until week 5 (3.30 W 0.60% vs 1.12 W 0.30%, P < 0.05). Animals fed on a high-fat diet were glucose intolerant (13.3 W 1.3 vs 9.4 W 0.8 mM in animals fed on a standard chow diet, for 60 min glycemia after glucose challenge, P < 0.05). In control animals, DNL accounted for 10.9 W 1.0% of HTG, whereas in animals given the high-fat diet, the DNL contribution was significantly reduced to 1.0 W 0.2% (P < 0.01 relative to controls). In a separate study to determine the response of HTG to weaning from a high-fat diet, animals with raised HTG (3.33 W 0.51%) after 7days of a high-fat diet reverted to basal HTG concentrations (0.76 W 0.06%) after an additional 7 days of weaning on a standard chow diet. These studies show that, in healthy rats, HTG concentrations are acutely influenced by dietary lipid concentrations. Although the DNL contribution to HTG content is suppressed by a high-fat diet in adult Sprague-Dawley rats, this effect is insufficient to prevent overall increases in HTG concentrations.

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A carne da tilápia possui boas características organolépticas. Este peixe apresenta a carne sem espinhos intramusculares, de cor branca, textura firme, aspecto fibroso e suculento, sabor apreciável e elevado valor nutricional (SOUZA; MARANHÃO, 2001). Por não apresentar espinhos na forma de "Y" no seu filé, é apropriada para a filetagem. Possui um rendimento em filé de aproximadamente 33% e a comercialização é realizada quando os espécimes atingem de 350 a 1000 g, dependendo da sua utilização (ALBUQUERQUE; ZAPATA; ALMEIDA, 2004;BOSCOLO;HAYASHI;MEURER, 2002; FURUYA et al., 2001). A espécie é recomendada para o consumo fresco, desidratado, salgado ou defumado (LEONHARDT et al., 2006).Os peixes apresentam proteínas de elevado valor biológico e a gordura destaca-se pela composição em ácidos graxos de valor AbstractThe use of coconut meal may represent an alternative source to fish feeding. The purpose of this work was to assess the impact of the use of coconut meal in Nile tilapia feeding and on the nutritional value of the fish fillets. The experiment was conducted in a completely randomized design with 4 treatments (T1 -0%, T2 -10%, T3 -20%, and T4 -30% for the inclusion of coconut meal) with five repetitions. The proximate composition, cholesterol, and fatty acids were determined. There was a difference (p < 0.05) between the treatments T1 and T3 and between T2 and T3 with regard to moisture levels; between T2 and T3 with regard to lipids; T1 and all the other treatments with regard to carbohydrates, and finally between T1 and T2 and between T3 and T4 with regard to ash. The cholesterol level showed a significant gradual increase following the increase in the inclusion of coconut meal. There was also a difference (p < 0.05) between the treatments concerning the following fatty acids: C 10:0 ; C 12:0 ; C 14:0 ; C 18:1 ; C 18:3 (gama); C 20:3 , and C 22:6 , but the nutritional quality of the product was not affected.

The objective of this work was to evaluated the epiphytic micro flora of sugarcane (Saccharrum officinarum L.), fresh gliricidia (Gliricidia sepium) (GNE), wilted gliricidia (GE) and mixtures ensiled. This study was conducted at the Universidade Federal de Alagoas-UFAL, from January until December 2005. The experimental design was entirely randomized in outline [(2x3x4)+4)]. The sugarcane silages added with GNE or GE were maded in the referred percentages: 100/0; 75/25; 50/50 and 25/75. There were 28 treatments, each one with three replications. Silages were kept in experimental silos (plastic boxes), sealed with sailcloth and adhesive ribbon. These silos were maintained under controlled temperature, and humidity conditions and saved from rodent presence. The silos were opened at 15, 45, 90 and 120 days, and sampled. In relation to effects of