Root bark extract of Terminalia avicennioides was obtained by cool maceration with 750 ml n-hexane, chloroform, ethyl acetate and methanol, independently for 48 h using soxhlet extractor. ATCC 33591 standard strains of methicillin-resistant Staphylococcus aureus (MRSA) was used. Strain resistance to methicillin/oxacillin antibiotic was verified by a retest of its sensitivity to oxacillin antibiotic. The antimicrobial test and zone of inhibition were determined using the agar well diffusion method. The isolated bioactive fractions of the extract were subjected to FTIR and GC-MS analysis. Results revealed both fractions (TLb4 and TLb17) were both bacteriostatic and bactericidal. The growth of MRSA was inhibited at extract concentrations of 60, 120, 180 and 240 µg/ml, within the susceptible range of ≥ 14 mm, with a mean inhibitory zone sensitivity of 14 mm at 60 µg/ml, 15.76 mm at 120 µg/ml and 15.33 mm at 180 µg/ml for fraction TLb4 and 15.33 mm at 60 µg/ml, 17.33 mm at 120 µg/ml and 20 mm at 180 µg/ml for TLb17 (≥ 14 mm). GC-MS detected oleic acid and analogs of palmitic acid as pharmacological active compounds of both fractions. FTIR showed the presence of alkyl halides. These bioactive agents revealed could be effective therapeutic agents for the treatment of MRSA.

Decitabine is a cytidine deoxynucleoside analog, which acts by inhibiting DNA methyltransferase, and is used for the treatment of acute myeloid leukemia. Decitabine has a short half-life (25 minutes), and is sensitive to harsh conditions. Elastic liposomes are an effective tool that can be used to overcome this disadvantage. Elastic liposomes also known as transfersomes are modified lipid carriers that enable drug to reach deeper skin layers and/or the systemic circulation. These vesicular formulations are several orders of magnitudes, more deformable than the standard liposomes and thus well suited for skin penetration. The objective of present study is to develop and evaluate the elastic liposomes of Decitabine so as to provide the sustained release and improve its bioavailability. Elastic liposomes were prepared by rotary evaporation method using Span 80 and Span 60 as a surfactants. The prepared Elastic liposomes were evaluated for entrapment efficiency, vesicle size, in vitro drug release. The drug release profiles from different elastic liposomes-in-vehicle formulations were in agreement with the physicochemical properties of the formulations. Based on different parameters formulations of batch ELS1 was found to be the best formulations. Stability study was performed on the selected formulation ELS1. Study concludes that Decitabine can also be formulated in the liposomal carrier which finds its best way for the topical administration.