No abstract

Cardiotoxicity is the most serious side effect of anthracyclines (doxorubicin, daunorubicin or epirubicin). The incidence of anthracycline induced late cardiac toxicity (AIC) that is overt clinically is 3-5% in the Indian population. Polymorphism in intron 32 (deletion of 25bp) of MYBPC3 has been shown to be present exclusively in Asians and more so in South India (3-8%). The frequency of the polymorphism is significantly higher (13%) in patients with cardiomyopathy in India. Fifteen patients were identified to have cardiac dysfunction following treatment for malignant lymphoma with doxorubicin containing regimens. Peripheral blood DNA from control, amplified by polymerase chain reaction yielded a 467bp fragment while in the presence of the 25bp deletion only a 442bp fragment was detected. To confirm the presence or absence of the polymorphism, amplified DNA was restricted using Bgl1 in all samples. Bgl1 restricted amplified DNA only if the 25bp deletion was absent. A 467 base pair band was observed in all the 15 samples, which suggested the absence of polymorphism in MYBPC3. In a sample of DNA from a patient with a deletion in exon 33 (confirmed by sequencing) a 442bp fragment was detected. Amplified DNA from this patient was not restricted with Bgl1. Wild type MYBPC3 when amplified gave a distinct restriction banding pattern consisting of two bands of 401bp and 66bp. Amplified DNA from all peripheral blood samples restricted with Bgl1 suggesting the absence of the polymorphism. In this preliminary report, MYBPC3 does not seem to play a role in anthracycline induced cardiotoxicity.

No abstract

No abstract

Introduction: Dental caries are not due to a single organism, but to complex interactions among multiple microbes found in the oral cavity. Microbiome studies have identified multiple organisms associated with dental caries in both the saliva and dental plaque, but taxa identified vary largely by study. Our scoping review aims to create a comprehensive list of cariogenic and prohealth taxa found in saliva and dental plaque among healthy children and adults that compare caries-active and caries-free populations. Methods: We searched published studies querying the PUBMED and EMBASE databases using the following keywords: (plaque OR saliva) AND caries AND (next generation sequencing OR checkerboard OR 16s rRNA or qPCR). Studies were limited to human studies published in English between January 1, 2010 and June 24, 2020.Results: Our search strategy identified 298 identified articles. After applying the exclusion criteria, 22 articles were included (Figure 1, Table 1 and 2). Taxa associated with caries or health varied widely among the studies reviewed, with notable differences by age and biologic sample type. While no single taxa was associated with caries in all studies, Streptococcus mutans was significantly associated with caries in 12/24 studies (50%) and Fusobacterium periodonticum was significantly associated with prohealth in 4/24 studies (16.7%). Conclusion: No taxa in plaque and salivary microbiomes were consistently associated with caries or prohealth across all studies. This may be due to the inconsistency of timing of sample collection during the caries process, differing sequencing methods, lack of correction for multiple testing or possibly indicate that there are multiple ways that the oral microbiome can be cariogenic or prohealth.